Syhre Mona, Scotter Jennifer M, Chambers Stephen T
Otago University, Christchurch School of Medicine and Health Sciences, Christchurch, New Zealand.
Med Mycol. 2008 May;46(3):209-15. doi: 10.1080/13693780701753800.
Our objective was to identify, for diagnostic purposes, potential volatile biomarkers of human microbial pathogens. We analysed the head space of cultures of medically important bacterial and fungal respiratory pathogens for 2-Pentylfuran (2PF) production through the use of Solid Phase Micro Extraction (SPME) and Gas Chromatography/Mass Spectroscopy (GC/MS). Our results confirm that 2PF is consistently produced by Aspergillus fumigatus, Fusarium spp., Aspergillus terreus, Aspergillus flavus and to a lesser extent by Aspergillus niger. 2-Pentylfuran was not detected from most of the bacterial strains except for Streptococcus pneumoniae. In human studies, four litre breath samples were collected from patients with cystic fibrosis (CF), with and without colonisation by A. fumigatus and other pathogens, as well as healthy volunteers. 2-Pentylfuran was detected in breath samples collected from 4/4 patients with CF and A. fumigatus colonization, 3/7 patients with CF and no microbiological evidence of A. fumigatus and 0/10 healthy control individuals. These results suggest that 2PF may be a biomarker for lung colonization/infection by fungal pathogens. To our knowledge, this is the first report describing the detection in breath samples of a volatile biomarker of a pathogen resident in the lungs. Breath analysis has the potential of being a non-invasive diagnostic method of detecting respiratory infection including invasive aspergillosis.
我们的目标是为诊断目的识别人类微生物病原体潜在的挥发性生物标志物。我们通过固相微萃取（SPME）和气相色谱/质谱联用（GC/MS）分析了具有医学重要性的细菌和真菌呼吸道病原体培养物的顶空，以检测2-戊基呋喃（2PF）的产生。我们的结果证实，烟曲霉、镰刀菌属、土曲霉、黄曲霉能持续产生2PF，黑曲霉产生量较少。除肺炎链球菌外，大多数细菌菌株均未检测到2-戊基呋喃。在人体研究中，从患有囊性纤维化（CF）且有或没有烟曲霉及其他病原体定植的患者以及健康志愿者中采集了4升呼气样本。在4/4例有烟曲霉定植的CF患者、3/7例无烟曲霉微生物学证据的CF患者的呼气样本中检测到了2-戊基呋喃，而在10例健康对照个体中未检测到。这些结果表明，2PF可能是真菌病原体肺部定植/感染的生物标志物。据我们所知，这是第一份描述在呼气样本中检测到肺部常驻病原体挥发性生物标志物的报告。呼气分析有可能成为一种检测包括侵袭性曲霉病在内的呼吸道感染的非侵入性诊断方法。
