Parrington M A, Lee P W, Kang C Y
Department of Microbiology and Immunology, University of Ottawa, Faculty of Medicine, Ontario, Canada.
J Gen Virol. 1991 Aug;72 ( Pt 8):1845-54. doi: 10.1099/0022-1317-72-8-1845.
Complementary DNA representing the genomic M RNA segment of the Prospect Hill (PH) Hantavirus was cloned and its nucleotide sequence determined. The PH virus M RNA segment is 3707 nucleotides in length and has a long open reading frame in the viral complementary-sense RNA with a coding capacity of 1142 amino acids. The predicted gene product of the PH virus M segment was compared with the corresponding gene products of Hantaan virus strain 76-118 (Hantaan), Sapporo rat virus strain SR-11 (SR) and Puumala virus strain Hällnäs B1 (Hällnäs). The amino acid sequence identities between the G1 and G2 proteins of PH virus and Hällnäs virus are respectively 74% and 79%. In contrast, the amino acid sequence similarities between the G1 proteins of PH virus and SR virus or Hantaan virus are only 50%. However the G2 proteins of SR and Hantaan viruses were more closely related to the G2 protein of PH virus with amino acid sequence similarities of approximately 62%. The G1 proteins of all four viruses had three potential asparagine-linked glycosylation sites conserved and there was one conserved site in the G2 proteins. Hydrophilicity plots of the four virus glycoproteins were very similar. The region of greatest hydrophilicity was conserved in the Hällnäs, SR and Hantaan viruses, and was located near the C terminus of the G1 protein, whereas the region of greatest hydrophilicity in the PH virus glycoprotein precursor is located closer to the N terminus of the G1 protein. Our data demonstrate that despite differences in the serotypic profiles and virulence of PH and Hällnäs viruses, their G1 and G2 proteins are closely related. We conclude that PH and Hällnäs viruses may have evolved along a separate evolutionary pathway in the Hantavirus genus from that of SR and Hantaan viruses.
代表展望山（PH）汉坦病毒基因组M RNA片段的互补DNA被克隆，并测定了其核苷酸序列。PH病毒M RNA片段长度为3707个核苷酸，在病毒互补义RNA中有一个长开放阅读框，编码能力为1142个氨基酸。将PH病毒M片段的预测基因产物与汉滩病毒76-118株（汉滩）、札幌大鼠病毒SR-11株（SR）和普马拉病毒Hällnäs B1株（Hällnäs）的相应基因产物进行了比较。PH病毒与Hällnäs病毒的G1和G2蛋白之间的氨基酸序列同一性分别为74%和79%。相比之下，PH病毒与SR病毒或汉滩病毒的G1蛋白之间的氨基酸序列相似性仅为50%。然而，SR病毒和汉滩病毒的G2蛋白与PH病毒的G2蛋白关系更密切，氨基酸序列相似性约为62%。所有四种病毒的G1蛋白都有三个保守的潜在天冬酰胺连接糖基化位点，G2蛋白中有一个保守位点。四种病毒糖蛋白的亲水性图谱非常相似。在Hällnäs病毒、SR病毒和汉滩病毒中，最大亲水区是保守的，位于G1蛋白的C末端附近，而PH病毒糖蛋白前体中的最大亲水区更靠近G1蛋白的N末端。我们的数据表明，尽管PH病毒和Hällnäs病毒在血清型谱和毒力方面存在差异，但其G1和G2蛋白密切相关。我们得出结论，PH病毒和Hällnäs病毒可能在汉坦病毒属中沿着与SR病毒和汉滩病毒不同的进化途径进化。
