da S Ferrão-Filho Aloysio, Soares Maria Carolina S, de Freitas Magalhães Valeria, Azevedo Sandra M F O
Laboratório de Avaliação e Promoção da Saúde Ambiental, Departamento de Biologia, Instituto Oswaldo Cruz, FIOCRUZ, Av. Brasil 4365, Manguinhos, Rio de Janeiro, RJ-CEP 21045-900, Brazil.
Ecotoxicol Environ Saf. 2009 Feb;72(2):479-89. doi: 10.1016/j.ecoenv.2008.02.002. Epub 2008 Apr 14.
This study evaluates the potential for the use of cladocerans in biomonitoring of cyanobacterial toxins. Two zooplankton species (Daphnia gessneri and Moina micrura) were cultivated in the laboratory for use in acute (48 h) and chronic (10 days) bioassays. Water samples were collected from two reservoirs and diluted in mineral water at four concentrations. Survivorship in the acute bioassays was used to calculate LC50, and survivorship and fecundity in chronic bioassays were used to calculate the intrinsic population growth rate (r) and the EC50. Analysis of phytoplankton in the water samples from one reservoir revealed that cyanobacteria were the dominant group, represented by the genera Anabaena, Cylindrospermopsis, and Microcystis. Results of bioassays showed adverse effects including death, paralysis, and reduced population growth rate, generally proportional to the reservoir water concentration. These effects may be related to the presence of cyanobacteria toxins (microcystins or saxitoxins) in the water.
本研究评估了枝角类用于蓝藻毒素生物监测的潜力。在实验室中培养了两种浮游动物（盖氏水蚤和微型裸腹溞），用于急性（48小时）和慢性（10天）生物测定。从两个水库采集水样，并以四种浓度在矿泉水中稀释。急性生物测定中的存活率用于计算半数致死浓度（LC50），慢性生物测定中的存活率和繁殖力用于计算内在种群增长率（r）和半数有效浓度（EC50）。对一个水库水样中的浮游植物分析表明，蓝藻是优势类群，以鱼腥藻属、柱孢藻属和微囊藻属为代表。生物测定结果显示出包括死亡、麻痹和种群增长率降低等不良影响，这些影响通常与水库水浓度成正比。这些影响可能与水中蓝藻毒素（微囊藻毒素或石房蛤毒素）的存在有关。
