Filon F Larese, D'Agostin F, Crosera M, Adami G, Rosani R, Romano C, Bovenzi M, Maina G
Unità Clinica Operativa di Medicina del Lavoro, Università degli Studi di Trieste .
G Ital Med Lav Ergon. 2007 Jul-Sep;29(3 Suppl):451-2.
There is a growing interest in the debate on nanoparticle safety for topical use. The benefits of nanoparticles have been shown in several scientific fields, but little is known about their potential to penetrate the skin lies. This study aims at evaluating in vitro silver nanoparticles skin penetration. Experiments were performed using the Franz diffusion cell method with intact and damaged human skin. Physiological solution was used as receiving phase and 70 microg/cm2 of silver nanoparticles dispersed in synthetic sweat were applied as donor phase to the outer surface of the skin for 24h. The receptor fluid measurements were performed by Electro Thermal Atomic Absorption Spectroscopy (ETAAS). Silver concentration of 0.2 microg/L was found in the receiving solutions of two cells, in which damaged skin membranes were set up. In the other tests, we obtained a silver concentration below the limit of detection in the receiving cells. Our experimental data show that silver nanoparticles permeation through intact and damaged skin is negligible. These findings are consistent with previously published results. Further researches are necessary to explore skin absorption of silver nanoparticles.
关于纳米颗粒局部使用安全性的争论正引发越来越多的关注。纳米颗粒的益处已在多个科学领域得到证实，但对于它们穿透皮肤的潜力却知之甚少。本研究旨在评估体外银纳米颗粒的皮肤渗透性。实验采用Franz扩散池法，使用完整和受损的人体皮肤。生理溶液用作接收相，将分散在合成汗液中的70微克/平方厘米银纳米颗粒作为供体相施加于皮肤外表面24小时。通过电热原子吸收光谱法（ETAAS）进行受体液测量。在设置了受损皮肤膜的两个细胞的接收溶液中发现银浓度为0.2微克/升。在其他测试中，我们在接收细胞中获得的银浓度低于检测限。我们的实验数据表明，银纳米颗粒透过完整和受损皮肤的渗透率可忽略不计。这些发现与先前发表的结果一致。有必要进行进一步研究以探索银纳米颗粒的皮肤吸收情况。
