Clinical Unit of Occupational Medicine, Department of Medical Sciences, University of Trieste, Via della Pietà 19, 34129 Trieste, Italy.
Toxicol In Vitro. 2013 Feb;27(1):121-7. doi: 10.1016/j.tiv.2012.09.007. Epub 2012 Sep 17.
Cobalt nanoparticles (CoNPs) are produced for several industrial and biomedical applications but there is a lack of data on human cutaneous absorption. Cobalt is also a skin sensitizer that can cause allergic contact dermatitis. Co applied as NPs, due to their small size and high surface, can penetrate into the skin in higher amount that bulk material. The aim of this study was to evaluate the absorption of Co applied as NPs in both intact and damaged skin. Experiments were performed using Franz cells and 1.0 mg cm(-2) of CoNPs was applied as donor phase for 24h. Mean Co content of 8.5 ± 1.2 ng cm(-2) and 1.87 ± 0.86 μg cm(-2) were found in the receiving solutions of Franz cells when the CoNPs suspension was applied on intact skin and on damaged skin, respectively. Twenty-four hours Co flux permeation was 76 ± 49 ng cm(-2)h(-1) in damaged skin with a lag time of 2.8 ± 2.1h. This study suggests that Co applied as NPs is able to penetrate the human skin in an in vitro diffusion cell system.
钴纳米粒子（CoNPs）被广泛应用于工业和生物医学领域，但有关其经皮吸收的数据却十分有限。钴同时也是一种皮肤致敏原，能够导致过敏性接触性皮炎。由于钴纳米粒子粒径小、比表面积大，与块状材料相比，其穿透皮肤的能力更强。本研究旨在评估 CoNPs 经皮吸收的情况，分别研究了完整皮肤和受损皮肤。实验采用 Franz 细胞扩散池，以 1.0mgcm(-2)的 CoNPs 作为供体相，应用 24 小时。当 CoNPs 混悬液应用于完整皮肤和受损皮肤时，Franz 细胞接收液中 Co 的平均含量分别为 8.5±1.2ngcm(-2)和 1.87±0.86μgcm(-2)。24 小时 Co 通量渗透为 76±49ngcm(-2)h(-1)，在有滞后时间的 2.8±2.1h 下，Co 穿透皮肤。本研究表明，CoNPs 能够在体外扩散细胞系统中穿透人类皮肤。
