Sunde Pia Titterud, Olsen Ingar, Enersen Morten, Beiske Klaus, Grinde Bjørn
Institute of Oral Biology, University of Oslo, Oslo, Norway.
J Med Virol. 2008 Jun;80(6):1007-11. doi: 10.1002/jmv.21180.
Periodontitis is presumably caused by bacterial infection, but it has been shown recently that affected tissue often contains human cytomegalovirus (HCMV) and Epstein-Barr virus (EBV). The present study was initiated to evaluate the role of these viruses in the pathogenesis of periodontitis. HCMV and EBV were quantified in 40 apical and 25 marginal periodontitis samples using real time PCR. In situ hybridization or immunohistochemistry was carried out on apical samples to detect viral presence within cells. A possible association with relevant bacteria was examined. Of the apical periodontitis samples, 50% contained EBV, while none contained HCMV. Of the marginal periodontitis samples, 40% were positive for EBV and 12% for HCMV. With one exception, however, the amount of virus was close to the detection limits. EBV was only detected in 1 out of 15 healthy periodontium samples. Immunohistochemistry and in situ hybridization were all negative. Significant associations were found between periodontal EBV and the presence of Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis. Although there was an obvious association of the virus with clinical samples, it seems unlikely that these viruses play a major role in the pathogenesis of periodontitis of the average patient. Their presence may reflect that the clinical samples contain more blood or saliva compared to controls, or an accumulation of lymphoid cells harboring virus in the inflamed tissue.
牙周炎可能由细菌感染引起，但最近研究表明，受影响的组织中常含有人类巨细胞病毒（HCMV）和爱泼斯坦 - 巴尔病毒（EBV）。本研究旨在评估这些病毒在牙周炎发病机制中的作用。使用实时PCR对40份根尖周炎样本和25份边缘性牙周炎样本中的HCMV和EBV进行定量分析。对根尖周炎样本进行原位杂交或免疫组织化学检测，以检测细胞内病毒的存在情况，并检查其与相关细菌的可能关联。在根尖周炎样本中，50%含有EBV，而无一例含有HCMV。在边缘性牙周炎样本中，40%的EBV呈阳性，12%的HCMV呈阳性。然而，除一例例外，病毒量接近检测限。在15份健康牙周组织样本中，仅1份检测到EBV，免疫组织化学和原位杂交均为阴性。牙周EBV与伴放线聚集杆菌和牙龈卟啉单胞菌的存在之间存在显著关联。尽管病毒与临床样本存在明显关联，但这些病毒似乎不太可能在普通患者牙周炎的发病机制中起主要作用。它们的存在可能反映出临床样本与对照相比含有更多血液或唾液，或者是炎症组织中携带病毒的淋巴细胞积聚。
