van Baren Marijke J, Koebbe Brian C, Brent Michael R
Washington University, St. Louis, Missouri.
Curr Protoc Bioinformatics. 2007 Dec;Chapter 4:4.8.1-4.8.16. doi: 10.1002/0471250953.bi0408s20.
N-SCAN is a gene-prediction system that combines the methods of ab initio predictors like GENSCAN with information derived from genome comparison. It is the latest in the TWINSCAN series of programs. This unit describes the use of N-SCAN to identify gene structures in eukaryotic genomic sequences. Protocols for using N-SCAN through its Web interface and from the command line in a Linux environment are provided. Detailed discussion about the appropriate parameter settings, input-sequence processing, and choice of genome for comparison are included.
N-SCAN是一种基因预测系统，它将诸如GENSCAN等从头预测器的方法与从基因组比较中获得的信息相结合。它是TWINSCAN系列程序中的最新版本。本单元描述了使用N-SCAN识别真核生物基因组序列中的基因结构。提供了通过其Web界面以及在Linux环境中从命令行使用N-SCAN的协议。其中包括有关适当参数设置、输入序列处理以及用于比较的基因组选择的详细讨论。
