Cagney Gerard, Uetz Peter
University of Toronto, Banting and Best Institute of Medical Research, Toronto, Ontario, Canada.
University of Washington, Seattle, Washington.
Curr Protoc Protein Sci. 2001 Aug;Chapter 19:19.6.1-19.6.12. doi: 10.1002/0471140864.ps1906s24.
Arrays are used for parallel assays of large numbers of proteins in a single experiment. They provide an alternative to libraries for protein screening purposes. This unit describes a protein array composed of living yeast (Saccharomyces cerevisiae) cells that can be used in a functional screen for protein interactions, the two-hybrid assay. The protocols described here use the yeast proteome as an example, but they can be applied to any other genome or subset thereof.
阵列用于在单个实验中对大量蛋白质进行平行分析。它们为蛋白质筛选目的提供了一种替代文库的方法。本单元描述了一种由活酵母（酿酒酵母）细胞组成的蛋白质阵列，可用于蛋白质相互作用的功能筛选，即双杂交分析。这里描述的方案以酵母蛋白质组为例，但它们可应用于任何其他基因组或其亚组。
