Fasolo Joseph, Snyder Michael
Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA.
Methods Mol Biol. 2009;548:209-22. doi: 10.1007/978-1-59745-540-4_12.
Protein microarrays containing nearly the entire yeast proteome have been constructed. They are typically prepared by overexpression and high-throughput purification and printing onto microscope slides. The arrays can be used to screen nearly the entire proteome in an unbiased fashion and have enormous utility for a variety of applications. These include protein-protein interactions, identification of novel lipid- and nucleic acid-binding proteins, and finding targets of small molecules, protein kinases, and other modification enzymes. Protein microarrays are thus powerful tools for individual studies as well as systematic characterization of proteins and their biochemical activities and regulation.
已经构建了包含几乎整个酵母蛋白质组的蛋白质微阵列。它们通常通过过表达、高通量纯化并打印到显微镜载玻片上来制备。这些阵列可用于以无偏倚的方式筛选几乎整个蛋白质组，并且在各种应用中具有巨大的实用性。这些应用包括蛋白质-蛋白质相互作用、鉴定新型脂质和核酸结合蛋白，以及寻找小分子、蛋白激酶和其他修饰酶的靶标。因此，蛋白质微阵列是用于个体研究以及蛋白质及其生化活性和调控的系统表征的强大工具。
