Altman Eleonora, Fernández Heriberto, Chandan Vandana, Harrison Blair A, Schuster Myra Wilson, Rademacher Laura Otth, Toledo Claudio
Institute for Biological Sciences, National Research Council of Canada, Ottawa, ON K1A 0R6, Canada.
Instituto de Microbiología Clínica, Edificio de Ciencias Biomédicas, Facultad de Medicina, Universidad Austral de Chile, PO Box 567, Valdivia, Chile.
J Med Microbiol. 2008 May;57(Pt 5):585-591. doi: 10.1099/jmm.0.47783-0.
Previous studies have shown that the LPS of Helicobacter pylori isolated from North American and European hosts predominantly expresses type 2 Lewis x (Le(x)) and Le(y) epitopes, whilst the LPS from Asian strains has the capacity to express type 1 Le(a) and Le(b) structures. The aim of this study was to evaluate the expression of Le antigens and the cytotoxin-associated antigen (CagA) by H. pylori isolates from Chile. A total of 38 isolates were screened. The expression of Le antigens and CagA was determined by whole-cell indirect ELISA, using commercially available monoclonal anti-Le and polyclonal anti-CagA antibodies. LPS profiles of H. pylori isolates were assessed by gel electrophoresis and Western blotting. Expression of Le(x) and/or Le(y) epitopes was confirmed in 32/38 isolates (84 %), whilst 9/38 isolates (24 %) expressed type 1 Le(b) blood group determinants, in addition to type 2 Le(x) and Le(y) structures. Six strains (16 %) were non-typeable. The majority of H. pylori strains examined were CagA-positive (83.3 %).
先前的研究表明，从北美和欧洲宿主分离出的幽门螺杆菌的脂多糖主要表达2型Lewis x（Le(x)）和Le(y)表位，而亚洲菌株的脂多糖能够表达1型Le(a)和Le(b)结构。本研究的目的是评估智利幽门螺杆菌分离株中Le抗原和细胞毒素相关抗原（CagA）的表达情况。共筛选了38株分离株。使用市售的抗Le单克隆抗体和抗CagA多克隆抗体，通过全细胞间接酶联免疫吸附测定法（ELISA）测定Le抗原和CagA的表达。通过凝胶电泳和蛋白质印迹法评估幽门螺杆菌分离株的脂多糖谱。在32/38株分离株（84%）中证实了Le(x)和/或Le(y)表位的表达，而9/38株分离株（24%）除了表达2型Le(x)和Le(y)结构外，还表达1型Le(b)血型决定簇。6株菌株（16%）无法分型。大多数检测的幽门螺杆菌菌株为CagA阳性（83.3%）。
