West China Marshall Research Center for Infectious Diseases, Center of Infectious Diseases, West China Hospital, Sichuan University, Chengdu, 610041, Sichuan, China.
Division of Infectious Diseases, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, 610041, Sichuan, China.
BMC Microbiol. 2023 Nov 22;23(1):360. doi: 10.1186/s12866-023-03116-0.
Helicobacter pylori lipopolysaccharide (LPS) structures vary among strains of different geographic origin. The aim of this study was to characterize the LPS O-antigen profiles of H. pylori strains isolated from Southwest China, and to further analyze the association of Lewis antigen expression with clinical outcomes and antibiotic resistance.
A total of 71 H. pylori isolates from Southwest China were included for LPS profiling by silver staining and Western blotting after SDS-PAGE electrophoresis. We demonstrated that all the clinical isolates had the conserved lipid A and core-oligosaccharide, whereas the O-antigen domains varied significantly among the isolates. Compared with the common presence of the glucan/heptan moiety in LPS O-antigen structure of European strains, the clinical isolates in this study appeared to lack the glucan/heptan moiety. The expression frequency of Le, Le, Le, and Le was 66.2% (47/71), 84.5% (60/71), 56.3% (40/71), and 31.0% (22/71), respectively. In total, the expression of type II Le and/or Le was observed in 69 (97.2%) isolates, while type I Le and/or Le were expressed in 49 (69.0%) isolates. No association of Lewis antigen expression with clinical outcomes or with antibiotic resistance was observed.
H. pylori strains from Southwest China tend to produce heptan-deficient LPS and are more likely to express type I Lewis antigens as compared with Western strains. This may suggest that H. pylori evolves to change its LPS structure for adaptation to different hosts.
幽门螺杆菌脂多糖（LPS）结构在不同地理来源的菌株中存在差异。本研究旨在对来自中国西南地区的幽门螺杆菌菌株的 LPS O-抗原谱进行特征分析，并进一步分析Lewis 抗原表达与临床结局和抗生素耐药性的关系。
共纳入 71 株来自中国西南地区的幽门螺杆菌分离株，通过 SDS-PAGE 电泳后的银染和 Western blot 分析 LPS 图谱。结果显示，所有临床分离株均具有保守的脂质 A 和核心寡糖，而 O-抗原结构域在分离株之间存在显著差异。与欧洲菌株 LPS O-抗原结构中常见的葡聚糖/庚烷部分相比，本研究中的临床分离株似乎缺乏葡聚糖/庚烷部分。Le、Le、Le 和 Le 的表达频率分别为 66.2%（47/71）、84.5%（60/71）、56.3%（40/71）和 31.0%（22/71）。总的来说，69 株（97.2%）分离株表达了 II 型 Le 和/或 Le，49 株（69.0%）分离株表达了 I 型 Le 和/或 Le。未观察到 Lewis 抗原表达与临床结局或与抗生素耐药性有关。
与西方菌株相比，来自中国西南地区的幽门螺杆菌菌株倾向于产生缺乏庚烷的 LPS，并且更可能表达 I 型 Lewis 抗原。这可能表明幽门螺杆菌为了适应不同的宿主而进化改变其 LPS 结构。
