Seixas Fernanda, Martins Maria da Luz, de Lurdes Pinto Maria, Travassos Paulo Jorge, Miranda Manuela, Pires Maria Dos Anjos
Laboratório de Histologia e Anatomia Patológica, Clínicas Veterinárias, Universidade de Trás-os-Montes e Alto Douro, 5001-801 Vila Real, Portugal.
J Wildl Dis. 2008 Apr;44(2):460-3. doi: 10.7589/0090-3558-44.2.460.
Pulmonary cryptococcosis was observed in a free-living adult female common toad (Bufo bufo) that was killed by a vehicle. Both lungs had various eosinophilic, monomorphic, and spherical to elliptical organisms identified as Cryptoccocus spp. The yeasts were demonstrated by Grocott's silver method and the periodic acid-Schiff reaction and the capsule was positive for mucin with a mucicarmine stain. The agent was confirmed by immunohistochemistry, using the monoclonal antibody anti-Cryptococcus neoformans, and by a polymerase chain reaction-based method using a C. neoformans-specific primer. This report, to the best of our knowledge, represents the first case of cryptococcosis in a common toad.
在一只被车辆撞死的成年野生雌性普通蟾蜍（Bufo bufo）中观察到肺隐球菌病。双肺可见多种嗜酸性、形态单一、球形至椭圆形的生物体，鉴定为隐球菌属。酵母通过格罗特银染色法、过碘酸-希夫反应显示，其荚膜经黏液卡红染色呈黏液阳性。通过使用抗新型隐球菌单克隆抗体的免疫组织化学方法以及使用新型隐球菌特异性引物的基于聚合酶链反应的方法对病原体进行了确认。据我们所知，本报告代表了普通蟾蜍中首例隐球菌病病例。
