Ballo Ahmed Mansour, Kokkari Anne K, Meretoja Ville V, Lassila Lippo L, Vallittu Pekka K, Narhi Timo O
Department of Prosthetic Dentistry and Biomaterials Science, Institute of Dentistry, University of Turku, Lemminkñisenkatu 2, Turku 20520, Finland.
J Mater Sci Mater Med. 2008 Oct;19(10):3169-77. doi: 10.1007/s10856-008-3453-y. Epub 2008 Apr 25.
The objective of this study was to evaluate the proliferation and osteogenic potential of bone-marrow derived osteoblast-like cells on fiber-reinforced composite (FRC) substrates with and without bioactive glass surface modification. Three FRC materials were fabricated for the study: (a) grit-blasted FRC, (b) grit-blasted FRC with bidirectional net reinforcement and (c) FRC with bioactive glass (BAG) coating. Rat bone-marrow derived osteoblast-like cells were harvested and cultured on experimental material plates and on cp. titanium plates (control) for 21 days. The materials' surfaces were characterized by roughness testing and scanning electron microscopy. Cell growth and differentiation kinetics were subsequently investigated by evaluating proliferation, alkaline phosphatase (ALP) activity, osteocalcin (OC) and bone sialoprotein (BSP) production. On day 14, the cell proliferation was significantly lower (P<0.05) on FRC-BAG than on titanium and FRC. The proliferation on the other three materials was equal throughout the experiment. The maximal ALP activities on FRC, FRC-Net, and titanium were observed on day 21, whereas FRC-BAG had already reached the maximal level on day 14. Expression of osteoblastic markers (OC, BSP) indicates that the fastest osteogenic differentiation takes place on FRC after 7 days. In contrast, a slower differentiation process was observed on titanium than on any other tested material (P<0.015) at 21 days, as was confirmed by increased mRNA expression of OC and BSP. It can be concluded that the proliferation and maturation of osteoblast-like cells on FRC appears to be comparable to titanium. Presence of BAG enhances cell maturation.
本研究的目的是评估骨髓来源的成骨样细胞在有和没有生物活性玻璃表面改性的纤维增强复合材料（FRC）基底上的增殖和成骨潜力。制备了三种FRC材料用于该研究：（a）喷砂处理的FRC，（b）双向网状增强的喷砂处理FRC，以及（c）具有生物活性玻璃（BAG）涂层的FRC。收获大鼠骨髓来源的成骨样细胞，并在实验材料板和对照钛板上培养21天。通过粗糙度测试和扫描电子显微镜对材料表面进行表征。随后通过评估增殖、碱性磷酸酶（ALP）活性、骨钙素（OC）和骨唾液蛋白（BSP）的产生来研究细胞生长和分化动力学。在第14天，FRC-BAG上的细胞增殖明显低于钛和FRC（P<0.05）。在整个实验过程中，其他三种材料上的增殖情况相同。FRC、FRC-Net和钛在第21天观察到最大的ALP活性，而FRC-BAG在第14天就已经达到了最大水平。成骨细胞标志物（OC、BSP）的表达表明，7天后FRC上的成骨分化最快。相比之下，在第21天观察到钛上的分化过程比任何其他测试材料都慢（P<0.015），OC和BSP的mRNA表达增加证实了这一点。可以得出结论，成骨样细胞在FRC上的增殖和成熟似乎与钛相当。BAG的存在增强了细胞成熟。
