Owczarzy Richard, Tataurov Andrey V, Wu Yihe, Manthey Jeffrey A, McQuisten Kyle A, Almabrazi Hakeem G, Pedersen Kent F, Lin Yuan, Garretson Justin, McEntaggart Neil O, Sailor Chris A, Dawson Robert B, Peek Andrew S
Department of Molecular Genetics and Biophysics, Integrated DNA Technologies, Inc., 1710 Commercial Park, Coralville, IA 52241, USA.
Nucleic Acids Res. 2008 Jul 1;36(Web Server issue):W163-9. doi: 10.1093/nar/gkn198. Epub 2008 Apr 25.
DNA and RNA oligomers are used in a myriad of diverse biological and biochemical experiments. These oligonucleotides are designed to have unique biophysical, chemical and hybridization properties. We have created an integrated set of bioinformatics tools that predict the properties of native and chemically modified nucleic acids and assist in their design. Researchers can select PCR primers, probes and antisense oligonucleotides, find the most suitable sequences for RNA interference, calculate stable secondary structures, and evaluate the potential for two sequences to interact. The latest, most accurate thermodynamic algorithms and models are implemented. This free software is available at http://www.idtdna.com/SciTools/SciTools.aspx.
DNA和RNA寡聚物被用于无数不同的生物学和生物化学实验中。这些寡核苷酸被设计成具有独特的生物物理、化学和杂交特性。我们创建了一套综合的生物信息学工具，可预测天然和化学修饰核酸的特性并辅助其设计。研究人员可以选择PCR引物、探针和反义寡核苷酸，找到最适合RNA干扰的序列，计算稳定的二级结构，并评估两个序列相互作用的可能性。实现了最新、最准确的热力学算法和模型。该免费软件可在http://www.idtdna.com/SciTools/SciTools.aspx获取。
