Mullane N, O'Gaora P, Nally J E, Iversen C, Whyte P, Wall P G, Fanning S
Centres for Food Safety & Food-borne Zoonomics, UCD Veterinary Sciences Centre, University College Dublin, Belfield, Dublin 4, Ireland.
Appl Environ Microbiol. 2008 Jun;74(12):3783-94. doi: 10.1128/AEM.02302-07. Epub 2008 Apr 25.
Nucleotide polymorphism associated with the O-antigen-encoding locus, rfb, in Enterobacter sakazakii was determined by PCR-restriction fragment length polymorphism analysis. Based on the analysis of these DNA profiles, 12 unique banding patterns were detected among a collection of 62 strains from diverse origins. Two common profiles were identified and were designated serotypes O:1 and O:2. DNA sequencing of the 12,500-bp region flanked by galF and gnd identified 11 open reading frames, all with the same transcriptional direction. Analysis of the proximal region of both sequences demonstrated remarkable heterogeneity. A PCR assay targeting genes specific for the two prominent serotypes was developed and applied for the identification of these strains recovered from food, environmental, and clinical samples.
通过聚合酶链反应-限制性片段长度多态性分析,确定了阪崎肠杆菌中与O抗原编码基因座rfb相关的核苷酸多态性。基于这些DNA图谱分析,在来自不同来源的62株菌株中检测到12种独特的条带模式。鉴定出两种常见模式,并指定为血清型O:1和O:2。对galF和gnd侧翼的12500 bp区域进行DNA测序,确定了11个开放阅读框,所有阅读框的转录方向相同。对两个序列近端区域的分析显示出显著的异质性。开发了一种针对两种主要血清型特异性基因的聚合酶链反应检测方法,并将其应用于从食品、环境和临床样本中分离出的这些菌株的鉴定。