Ling Na, Li Chengsi, Zhang Jumei, Wu Qingping, Zeng Haiyan, He Wenjing, Ye Yingwang, Wang Juan, Ding Yu, Chen Moutong, Xue Liang, Ye Qinghua, Guo Weipeng
School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, China.
State Key Laboratory of Applied Microbiology, South China, Guangdong Provincial Key Laboratory of Microbiology Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Guangzhou, China.
Front Microbiol. 2018 Jun 5;9:1149. doi: 10.3389/fmicb.2018.01149. eCollection 2018.
spp. is a foodborne pathogen that causes life-threatening and invasive diseases, such as necrotizing enterocolitis, meningitis, and sepsis. In this study, we aimed to investigate the prevalence, molecular characteristics and antimicrobial resistance of spp. in raw vegetables marketed in China. Based on dietary habits in China, 403 raw vegetables that could be eaten without additional cooking were collected. Of the 403 samples tested, 122 (30.27%) were positive for spp., and the contamination levels exceeded 110 most probable number (MPN)/g for 16.39% (20/122) of the samples. Coriander samples had the highest contamination rate of 52.81%, and the MPN values of 19.15% of positive coriander samples exceeded 100 MPN/g. Eleven serotypes were identified among 171 isolates, with serogroup O1 (41 isolates) being the dominant serotype. Molecular characterization indicated that there was quite high genetic diversity in spp., and multilocus sequence typing analyses yielded 106 sequence types (STs), 55 of which were newly identified. Notably, the most prevalent ST (eight isolates) was . ST60, which appeared in a recent clinical infectious disease study in China. Five ST4, seven . ST7, and three ST8 confirmed as pathogenic STs in other countries were also detected in this study. Furthermore, all isolates were susceptible to amikacin, amoxicillin-clavulanic, cefepime, ciprofloxacin, and imipenem, but some isolates exhibited a high ratio of resistance to cephalothin (59.65%). In this study, the high contamination rate and the detection of pathogenic and new STs in raw vegetables indicated potential hazards to customers. To the best of our knowledge, this is the first report to provide valuable information on the contamination status of spp. in vegetables that can be eaten raw in China.
[病原菌名称]是一种食源性病原体，可引发危及生命的侵袭性疾病，如坏死性小肠结肠炎、脑膜炎和败血症。在本研究中，我们旨在调查在中国市场销售的生蔬菜中[病原菌名称]的流行情况、分子特征及抗菌药物耐药性。根据中国的饮食习惯，收集了403种无需额外烹饪即可食用的生蔬菜。在检测的403个样本中，122个（30.27%）[病原菌名称]呈阳性，16.39%（20/122）的样本污染水平超过110最可能数（MPN）/克。香菜样本的污染率最高，为52.81%，19.15%的阳性香菜样本的MPN值超过100 MPN/克。在171株分离株中鉴定出11种血清型，其中O1血清群（41株）为优势血清型。分子特征表明，[病原菌名称]具有相当高的遗传多样性，多位点序列分型分析产生了106种序列类型（STs），其中55种是新鉴定的。值得注意的是，最常见的ST（8株分离株）是[具体ST编号]。ST60，在中国最近的一项临床传染病研究中出现过。本研究还检测到在其他国家被确认为致病STs的5株[病原菌名称] ST4、7株[病原菌名称] ST7和3株[病原菌名称] ST8。此外，所有分离株对阿米卡星、阿莫西林-克拉维酸、头孢吡肟、环丙沙星和亚胺培南敏感，但一些分离株对头孢噻吩的耐药率较高（59.65%）。在本研究中，生蔬菜的高污染率以及致病和新STs的检测表明对消费者存在潜在危害。据我们所知，这是第一份提供关于中国可生食蔬菜中[病原菌名称]污染状况有价值信息的报告。
需注意，原文中部分病原菌名称未明确给出，翻译时用[病原菌名称]代替，实际翻译时应根据准确的病原菌名称进行翻译。
