三氧化矿物凝聚体对小鼠牙髓组织细胞因子产生的影响。
The effects of mineral trioxide aggregates on cytokine production by mouse pulp tissue.
作者信息
Barbosa Silva Marcelo José, Vieira Leda Quercia, Sobrinho Antônio Paulino Ribeiro
机构信息
Department of Biochemistry and Immunology, Institute of Biological Science, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.
出版信息
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2008 May;105(5):e70-6. doi: 10.1016/j.tripleo.2008.01.025.
OBJECTIVE
The objective of this study was to determine the effect of MTA on the expression of cytokines in mouse pulp tissue.
STUDY DESIGN
Pulp tissue was exposed to MTA, and the expression of CCL5/RANTES, CCL2/MCP1, IL-1alpha, IFN-gamma, TNF-alpha, IL-4, and IL-6 was evaluated by RT-PCR at 10 and 20 days after exposure. Control groups were not exposed to MTA.
RESULTS
We found no detectable expression of CCL2, IL-4, and IL-6 in the tissue from either group, while TNF-alpha was expressed at high levels 20 days after exposure (P < .05). CCL5 and IL-1alpha mRNA expression was lower in the MTA-treated group 10 days after treatment (P < .05). At 20 days after the surgical procedure, IFN-gamma mRNA expression was also lower in the MTA-treated group (P < .05).
CONCLUSIONS
These findings suggest that MTA down-regulates the inflammatory cytokines CCL5, IL-1alpha and IFN-gamma and may have an anti-inflammatory effect.
目的
本研究的目的是确定矿物三氧化物凝聚体（MTA）对小鼠牙髓组织中细胞因子表达的影响。
研究设计
将牙髓组织暴露于MTA，在暴露后10天和20天通过逆转录聚合酶链反应（RT-PCR）评估趋化因子配体5/调节激活正常T细胞表达和分泌因子（CCL5/RANTES）、趋化因子配体2/单核细胞趋化蛋白1（CCL2/MCP1）、白细胞介素-1α（IL-1α）、干扰素-γ（IFN-γ）、肿瘤坏死因子-α（TNF-α）、白细胞介素-4（IL-4）和白细胞介素-6（IL-6）的表达。对照组未暴露于MTA。
结果
我们发现两组组织中均未检测到CCL2、IL-4和IL-6的表达，而TNF-α在暴露后20天高水平表达（P <.05）。处理后10天，MTA处理组中CCL5和IL-1α信使核糖核酸（mRNA）表达较低（P <.05）。手术操作后20天，MTA处理组中IFN-γ mRNA表达也较低（P <.05）。
结论
这些发现表明MTA下调炎症细胞因子CCL5、IL-1α和IFN-γ，可能具有抗炎作用。
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