培养用三氧化矿物凝聚体或硅酸钙水门汀的牙髓细胞中炎症标志物环氧化酶-2 的表达。
Expression of the inflammatory marker cyclooxygenase-2 in dental pulp cells cultured with mineral trioxide aggregate or calcium silicate cements.
机构信息
Institute of Oral Biology and Biomaterials, Chung Shan Medical University, and Dental Department, Chung Shan Medical University Hospital, Taichung, Taiwan.
出版信息
J Endod. 2010 Mar;36(3):465-8. doi: 10.1016/j.joen.2009.12.008.
INTRODUCTION
Mineral trioxide aggregate (MTA) and calcium silicate (CS) cements exhibit acceptable physical and chemical properties. The aim of the present study was to evaluate the effects of MTA and CS cements on inflammatory reactions in primary cultured human dental pulp cells.
METHODS
The mitochondrial colorimetric assay was used to evaluate pulp cell survival rates. Fluorescent immunohistochemistry was used to observe focal adhesion kinase (FAK) and cyclooxygenase-2 (COX-2) distributions in the cells. Reverse transcription-polymerase chain reaction was used to assess COX-2 expression.
RESULTS
The results showed that MTA and CS are biocompatible with pulp cells (P>.05). FAK was well-distributed in pulp cells in contact with both cements. Both MTA and CS cements induced pulp cell inflammation as evidenced by increased COX-2 expression.
CONCLUSIONS
The present study demonstrated that MTA and CS cements are biocompatible with primary cultured pulp cells. Both cements can induce inflammatory COX-2 expression in the pulp cells.
简介
矿化三氧化物凝聚体（MTA）和硅酸钙（CS）水泥表现出可接受的物理和化学性能。本研究旨在评估 MTA 和 CS 水泥对原代培养人牙髓细胞炎症反应的影响。
方法
采用线粒体比色法评估牙髓细胞存活率。荧光免疫组织化学观察细胞中粘着斑激酶（FAK）和环氧化酶-2（COX-2）的分布。逆转录聚合酶链反应评估 COX-2 的表达。
结果
结果表明，MTA 和 CS 与牙髓细胞具有生物相容性（P>.05）。FAK 在与两种水泥接触的牙髓细胞中分布良好。MTA 和 CS 水泥均可诱导牙髓细胞炎症，表现为 COX-2 表达增加。
结论
本研究表明，MTA 和 CS 水泥与原代培养的牙髓细胞具有生物相容性。两种水泥均可诱导牙髓细胞中炎症 COX-2 的表达。
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