Du Zhen-Yu, Clouet Pierre, Degrace Pascal, Zheng Wen-Hui, Frøyland Livar, Tian Li-Xia, Liu Yong-Jian
Institute of Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, 135 Xin'gang Xi Road, 510275 Guangzhou, China.
Br J Nutr. 2008 Dec;100(6):1200-12. doi: 10.1017/S0007114508986840. Epub 2008 Apr 29.
We investigated whether the hypolipidaemic effect of fenofibrate and fasting observed in most omnivorous mammals may also apply to herbivorous fish. Grass carp (Ctenopharyngodon idella) fed a high-fat (8 %) diet exhibited a marked increase in blood lipids and body fat after 6 weeks. They were then treated with fenofibrate (100 mg/kg body weight) in the same high-fat diet for 2 weeks, followed by fasting for 1 week. Plasma lipid concentration, body fat amount, fatty acid composition, plasma thiobarbituric acid-reactive substances and some parameters related to hepatic fatty acid oxidation were measured, and liver samples were stained for histological examination. Fenofibrate treatment decreased TAG and cholesterol concentrations in plasma, total lipids of the whole body and liver, and EPA and DHA contents in tissues. Further, a mobilisation of mesenteric fat concomitant with an increase in hepatic peroxisomal fatty acid oxidation and lipid peroxidation was observed. Compared with fenofibrate treatment, fasting decreased body weight and plasma TAG, but not plasma cholesterol. It also reduced the fat content of the whole body and increased the EPA and DHA contents in the liver and other tissues. Fatty acid oxidation was stimulated by fasting in mitochondria, but not in peroxisomes. These data suggest that fenofibrate and fasting regulate the lipid metabolism in grass carp through different metabolic pathways. The grass carp is moderately responsive to a fibrate derivative in comparison with the well-known excess responsiveness of the rat model, and so it could be used for the study of lipid abnormalities as a herbivorous model.
我们研究了在大多数杂食性哺乳动物中观察到的非诺贝特和禁食的降血脂作用是否也适用于草食性鱼类。喂食高脂（8%）日粮的草鱼（Ctenopharyngodon idella）在6周后血脂和体脂显著增加。然后，它们在相同的高脂日粮中接受非诺贝特（100毫克/千克体重）处理2周，随后禁食1周。测量血浆脂质浓度、体脂量、脂肪酸组成、血浆硫代巴比妥酸反应性物质以及一些与肝脏脂肪酸氧化相关的参数，并对肝脏样本进行染色以进行组织学检查。非诺贝特处理降低了血浆中的甘油三酯和胆固醇浓度、全身和肝脏的总脂质以及组织中的二十碳五烯酸（EPA）和二十二碳六烯酸（DHA）含量。此外，观察到肠系膜脂肪的动员伴随着肝脏过氧化物酶体脂肪酸氧化和脂质过氧化的增加。与非诺贝特处理相比，禁食降低了体重和血浆甘油三酯，但没有降低血浆胆固醇。它还降低了全身的脂肪含量，并增加了肝脏和其他组织中的EPA和DHA含量。禁食刺激了线粒体中的脂肪酸氧化，但未刺激过氧化物酶体中的脂肪酸氧化。这些数据表明，非诺贝特和禁食通过不同的代谢途径调节草鱼的脂质代谢。与众所周知的大鼠模型的过度反应性相比，草鱼对贝特类衍生物的反应适中，因此它可作为草食性模型用于脂质异常的研究。
