Cuba-Cuba C A, Evans D, Rosa A de C, Marsden P D
University of Brasília, DF, Brazil.
Rev Inst Med Trop Sao Paulo. 1991 Sep-Oct;33(5):343-50. doi: 10.1590/s0036-46651991000500002.
Three isolates over 5 years from a patient with persistent relapsing mucosal leishmaniasis due to Leishmania (Viannia) braziliensis and 7 clones from one of these isolates were studied by zymodemes and serodemes analysis. Results showed evidences of clonal phenotypic variation. Eight isoenzymes markers demonstrated clear differences on Cellulose Acetate (CA) and thin starch gel electrophoresis. Also a panel of specific monoclonal antibodies showed such differences. Our observations provide additional evidence that Leishmania (Viannia) braziliensis is composed by subpopulations of parasites with peculiar biochemical and antigenic characteristics.
对一名因巴西利什曼原虫（维安尼亚利什曼原虫）感染导致黏膜利什曼病持续复发的患者在5年期间分离出的3株菌株，以及其中1株菌株的7个克隆进行了酶谱型和血清型分析。结果显示存在克隆表型变异的证据。8种同工酶标记物在醋酸纤维素（CA）和薄淀粉凝胶电泳上表现出明显差异。一组特异性单克隆抗体也显示出此类差异。我们的观察结果提供了额外证据，表明巴西利什曼原虫（维安尼亚利什曼原虫）由具有独特生化和抗原特性的寄生虫亚群组成。
