乌宗湖嗜碱热放线菌新种的描述及嗜碱热放线菌属的修订描述。
Description of Caldalkalibacillus uzonensis sp. nov. and emended description of the genus Caldalkalibacillus.
作者信息
Zhao Weidong, Zhang Chuanlun L, Romanek Christopher S, Wiegel Juergen
机构信息
Department of Marine Sciences, University of Georgia, Athens, GA 30602, USA.
出版信息
Int J Syst Evol Microbiol. 2008 May;58(Pt 5):1106-8. doi: 10.1099/ijs.0.65363-0.
Strain JW/WZ-YB58T, a thermophilic (42-64 degrees C), aerobic, alkalitolerant (pH25 degrees C 6.4-9.7), heterotrophic, sporulating, retarded-peritrichously flagellated and slightly curved rod-shaped bacterium, was isolated from the hot spring Zarvarzin II in the East Thermal Field of the Uzon Caldera, Kamchatka (Far East Russia). The isolate tolerated high concentrations of CO. The major membrane phospholipid fatty acids of JW/WZ-YB58T included iso-C15 : 0 (24.5 %), anteiso-C15 : 0 (18.3 %) and iso-C17 : 0 (17.5 %). The G+C content of the genomic DNA is 45 mol% (HPLC method). Based on 16S rRNA gene sequence analysis and physiological properties, isolate JW/WZ-YB58T (=ATCC BAA-1258T =DSM 17740T) is proposed as the type strain of Caldalkalibacillus uzonensis sp. nov. In contrast to the type species Caldalkalibacillus thermarum, a catalase-reaction-positive aerobe, C. uzonensis was catalase-reaction-negative; thus the description of the genus Caldalkalibacillus is emended to include a catalase-reaction-negative species.
菌株JW/WZ-YB58T是一种嗜热菌（42-64摄氏度），好氧，耐碱（25摄氏度时pH值为6.4-9.7），异养，产芽孢，周生鞭毛生长缓慢且呈微弯杆状的细菌，从堪察加半岛乌宗火山口东热区的扎尔瓦尔津二号温泉中分离得到（俄罗斯远东地区）。该分离株耐受高浓度的一氧化碳。JW/WZ-YB58T的主要膜磷脂脂肪酸包括异-C15 : 0（24.5%）、anteiso-C15 : 0（18.3%）和异-C17 : 0（17.5%）。基因组DNA的G+C含量为45 mol%（高效液相色谱法）。基于16S rRNA基因序列分析和生理特性，分离株JW/WZ-YB58T（=ATCC BAA-1258T =DSM 17740T）被提议作为乌宗温泉嗜碱芽孢杆菌新种的模式菌株。与模式种嗜热嗜碱芽孢杆菌（一种过氧化氢酶反应阳性的需氧菌）不同，乌宗温泉嗜碱芽孢杆菌过氧化氢酶反应阴性；因此，嗜碱芽孢杆菌属的描述被修订为包括一种过氧化氢酶反应阴性的物种。
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