Laboratoire de Microbiologie et Biotechnologie des Environnements Chauds, UMR_D180, IRD, Universités de Provence et de la Méditerranée, ESIL, case 925, 163 avenue de Luminy, 13288, Marseille Cedex 9, France.
Extremophiles. 2010 May;14(3):297-304. doi: 10.1007/s00792-010-0308-5. Epub 2010 Apr 1.
A novel thermophilic anaerobic and microaerophilic bacterium (optimal growth in the presence of 5-10% O(2)), strain Nad S1(T) was isolated from the terrestrial hot spring of Hammam Sidi Jdidi, Nabeul, Tunisia. Cells were motile rods having a Gram-positive cell wall structure. Strain Nad S1(T) grew optimally at 55 degrees C (range 37-70 degrees C). Optimum pH for growth was 6.5-7.0. It was halotolerant growing with NaCl up to 7% (optimum concentration 1.5-3.0%). It grew chemoorganotrophically on various carbohydrates, organic-acids and amino-acids as energy sources, or chemolithotrophically on H(2) using nitrate, as terminal electron acceptor. Beside oxygen (under microaerobic conditions) and nitrate, nitrite was also used. Nitrate was completely reduced to N(2). No fermentation occurred. The genomic DNA G + C content was 41.8 mol%. Based on 16S rRNA gene sequence analysis, strain Nad S1(T) belongs to the Bacillaceae family within the class 'Bacilli'. Because of its phylogenetic and phenotypic characteristics, we propose this isolate to be assigned as a novel genus and a novel species within the domain Bacteria, Microaerobacter geothermalis gen. nov., sp. nov. The type strain is Nad S1(T) (=DSM 22679(T) =JCM 16213(T)).
一株新型嗜热厌氧兼微好氧细菌（在 5-10%氧气存在下最佳生长），菌株 Nad S1(T)，从突尼斯纳布勒的 Hammam Sidi Jdidi 陆地温泉中分离得到。细胞是能动的杆状，具有革兰氏阳性细胞壁结构。菌株 Nad S1(T)在 55°C 下最佳生长（范围为 37-70°C）。生长的最佳 pH 值为 6.5-7.0。它具有耐盐性，可在高达 7%的 NaCl 中生长（最佳浓度为 1.5-3.0%）。它可以异养生长，以各种碳水化合物、有机酸和氨基酸作为能源，或自养生长，以氢气为能源，以硝酸盐作为末端电子受体。除了氧气（在微氧条件下）和硝酸盐外，亚硝酸盐也被使用。硝酸盐完全还原为 N2。没有发酵发生。基因组 DNA G+C 含量为 41.8mol%。基于 16S rRNA 基因序列分析，菌株 Nad S1(T)属于 Bacillaceae 科，属于“芽孢杆菌”类。由于其系统发育和表型特征，我们建议将该分离株分配为一个新属和一个新种，属于细菌域，名为 Microaerobacter geothermalis 属，种。模式株是 Nad S1(T)（=DSM 22679(T)=JCM 16213(T)）。
