Merz Katharina, Hondele Maria, Goetze Hannah, Gmelch Katharina, Stoeckl Ulrike, Griesenbeck Joachim
Universitaet Regensburg, Institut für Biochemie, Genetik und Mikrobiologie, 93053 Regensburg, Germany.
Genes Dev. 2008 May 1;22(9):1190-204. doi: 10.1101/gad.466908.
Synthesis of ribosomal RNAs (rRNAs) is the major transcriptional event in proliferating cells. In eukaryotes, ribosomal DNA (rDNA) is transcribed by RNA polymerase I from a multicopy locus coexisting in at least two different chromatin states. This heterogeneity of rDNA chromatin has been an obstacle to defining its molecular composition. We developed an approach to analyze differential protein association with each of the two rDNA chromatin states in vivo in the yeast Saccharomyces cerevisiae. We demonstrate that actively transcribed rRNA genes are largely devoid of histone molecules, but instead associate with the high-mobility group protein Hmo1.
核糖体RNA（rRNA）的合成是增殖细胞中的主要转录事件。在真核生物中，核糖体DNA（rDNA）由RNA聚合酶I从至少两种不同染色质状态共存的多拷贝基因座转录而来。rDNA染色质的这种异质性一直是确定其分子组成的障碍。我们开发了一种方法，用于在酿酒酵母体内分析与两种rDNA染色质状态各自相关的差异蛋白质。我们证明，活跃转录的rRNA基因基本上没有组蛋白分子，而是与高迁移率族蛋白Hmo1相关联。
