Kasahara Koji, Ohtsuki Kazushige, Ki Sewon, Aoyama Kayo, Takahashi Hiroyuki, Kobayashi Takehiko, Shirahige Katsuhiko, Kokubo Tetsuro
Division of Molecular and Cellular Biology, Science of Supramolecular Biology, International Graduate School of Arts and Sciences, Yokohama City University, Yokohama, Kanagawa, Japan.
Mol Cell Biol. 2007 Oct;27(19):6686-705. doi: 10.1128/MCB.00876-07. Epub 2007 Jul 23.
HMO1 is a high-mobility group B protein that plays a role in transcription of genes encoding rRNA and ribosomal proteins (RPGs) in Saccharomyces cerevisiae. This study uses genome-wide chromatin immunoprecipitation to study the roles of HMO1, FHL1, and RAP1 in transcription of these genes as well as other RNA polymerase II-transcribed genes in yeast. The results show that HMO1 associates with the 35S rRNA gene in an RNA polymerase I-dependent manner and that RPG promoters (138 in total) can be classified into several distinct groups based on HMO1 abundance at the promoter and the HMO1 dependence of FHL1 and/or RAP1 binding to the promoter. FHL1, a key regulator of RPGs, binds to most of the HMO1-enriched and transcriptionally HMO1-dependent RPG promoters in an HMO1-dependent manner, whereas it binds to HMO1-limited RPG promoters in an HMO1-independent manner, irrespective of whether they are transcribed in an HMO1-dependent manner. Reporter gene assays indicate that these functional properties are determined by the promoter sequence.
HMO1是一种高迁移率族B蛋白，在酿酒酵母中对编码rRNA和核糖体蛋白（RPG）的基因转录起作用。本研究使用全基因组染色质免疫沉淀技术来研究HMO1、FHL1和RAP1在这些基因以及酵母中其他RNA聚合酶II转录基因转录过程中的作用。结果表明，HMO1以RNA聚合酶I依赖的方式与35S rRNA基因相关联，并且RPG启动子（总共138个）可根据启动子处的HMO1丰度以及FHL1和/或RAP1与启动子结合的HMO1依赖性分为几个不同的组。FHL1是RPG的关键调节因子，它以HMO1依赖的方式与大多数富含HMO1且转录依赖于HMO1的RPG启动子结合，而它以HMO1非依赖的方式与HMO1有限的RPG启动子结合，无论它们是否以HMO1依赖的方式转录。报告基因分析表明，这些功能特性由启动子序列决定。
