Srivastava Neena, Achyut B R, Prakash Jai, Agarwal C G, Pant D C, Mittal Balraj
Department of Physiology, Chatrapati Shahuji Maharaj Medical University, Lucknow, India.
Mol Cell Biochem. 2008 Jul;314(1-2):171-7. doi: 10.1007/s11010-008-9778-5. Epub 2008 May 4.
Background The pathophysiology of obesity is known to be influenced by alterations in lipid levels. We aimed to evaluate association of cholesteryl ester transfer protein (CETP) and apolipoprotein (APO) E gene variants with asymptomatic obesity. Methods A total of 437 subjects, 159 asymptomatic obese (BMI = 29.29 +/- 3.76) and 278 non-obese (BMI = 23.38 +/- 1.71) individuals, were included in this case-control study. Lipid levels were estimated using standard protocols. Analysis of CETP (TaqIB) and APOE (HhaI) gene polymorphisms was done using PCR-RFLP. Results We found significant difference in blood pressure (systolic, P < 0.0001 and diastolic, P < 0.0001), total cholesterol (P < 0.0001), LDL-cholesterol (P < 0.0001), and HDL-cholesterol (P < 0.0001) in obese as compared to non-obese group. Homozygous APO E4E4 genotype was only observed in 5.7% of obese individuals and none in non-obese group. APO E4 allele carriers were also susceptible for obesity (P = 0.016, OR = 1.73; 95% CI = 1.12-2.68) than non-carriers. Higher blood pressure (Systolic, P = 0.001 and Diastolic, P = 0.004) and triglyceride levels (P = 0.029) were observed in obese subjects with APO E4 allele than individuals without APO E4. However, CETP B1 variant allele carriers did not show alteration in blood pressure and lipid profile in asymptomatic obese subjects. Conclusions APO E4 genotype and allele were found to be associated with asymptomatic obesity, whereas CETP Taq1B polymorphism showed no such association in North Indian subjects.
背景 已知肥胖的病理生理学受血脂水平改变的影响。我们旨在评估胆固醇酯转运蛋白（CETP）和载脂蛋白（APO）E基因变异与无症状肥胖之间的关联。方法 本病例对照研究共纳入437名受试者，其中159名无症状肥胖者（BMI = 29.29±3.76）和278名非肥胖者（BMI = 23.38±1.71）。采用标准方案估算血脂水平。使用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）分析CETP（TaqIB）和APOE（HhaI）基因多态性。结果 与非肥胖组相比，我们发现肥胖组的血压（收缩压，P < 0.0001；舒张压，P < 0.0001）、总胆固醇（P < 0.0001）、低密度脂蛋白胆固醇（P < 0.0001）和高密度脂蛋白胆固醇（P < 0.0001）存在显著差异。纯合子APO E4E4基因型仅在5.7%的肥胖个体中观察到，非肥胖组中未观察到。APO E4等位基因携带者也比非携带者更容易患肥胖症（P = 0.016，OR = 1.73；95%CI = 1.12 - 2.68）。与没有APO E4等位基因的个体相比，携带APO E4等位基因的肥胖受试者的血压（收缩压，P = 0.001；舒张压，P = 0.004）和甘油三酯水平（P = 0.029）更高。然而，CETP B1变异等位基因携带者在无症状肥胖受试者中未表现出血压和血脂谱的改变。结论 发现APO E4基因型和等位基因与无症状肥胖有关，而在北印度受试者中，CETP Taq1B多态性未显示出这种关联。
