Stapleton J T, Lange D K, LeDuc J W, Binn L N, Jansen R W, Lemon S M
Department of Internal Medicine, VA Medical Center, Iowa City.
J Infect Dis. 1991 Jan;163(1):7-11. doi: 10.1093/infdis/163.1.7.
Because the role of intestinal immunity remains uncertain in hepatitis A, samples of feces and saliva from infected primates and humans were tested for virus neutralizing activity. Only two of eight owl monkeys infected by the intragastric route developed neutralizing antibody detectable in extracts of feces collected up to 88 days after viral challenge, although serum neutralizing antibody was present in all monkeys by day 33. Similarly, neutralizing antibody was detected in fecal extracts from none of three experimentally infected human volunteers and only 1 of 15 naturally infected humans. The single positive human specimen contained occult blood. Only 2 of 19 saliva samples from naturally infected humans had significant viral neutralizing activity. In contrast, neutralizing antibody to type 2 poliovirus was present in most human fecal or saliva specimens tested. These data suggest that intestinal immunity does not play a significant role in protection against hepatitis A.
由于肠道免疫在甲型肝炎中的作用仍不明确，因此对受感染的灵长类动物和人类的粪便及唾液样本进行了病毒中和活性检测。通过胃内途径感染的八只夜猴中，只有两只在病毒攻击后长达88天收集的粪便提取物中检测到可中和抗体，尽管到第33天时所有猴子的血清中都存在中和抗体。同样，在三名实验感染的人类志愿者的粪便提取物中均未检测到中和抗体，在15名自然感染的人类中仅1人检测到。唯一呈阳性的人类样本含有潜血。在19份自然感染人类的唾液样本中，只有两份具有显著的病毒中和活性。相比之下，在大多数检测的人类粪便或唾液样本中都存在针对2型脊髓灰质炎病毒的中和抗体。这些数据表明，肠道免疫在预防甲型肝炎方面并未发挥重要作用。
