乙酰化组蛋白H3和H4标记了淋巴样细胞中爱泼斯坦-巴尔病毒上调的LMP2A启动子。
Acetylated histone H3 and H4 mark the upregulated LMP2A promoter of Epstein-Barr virus in lymphoid cells.
作者信息
Gerle Borbala, Koroknai Anita, Fejer György, Bakos Agnes, Banati Ferenc, Szenthe Kalman, Wolf Hans, Niller Hans Helmut, Minarovits Janos, Salamon Daniel
机构信息
Microbiological Research Group, National Center for Epidemiology, Pihenö u. 1, H-1529 Budapest, Hungary.
出版信息
J Virol. 2007 Dec;81(23):13242-7. doi: 10.1128/JVI.01396-07. Epub 2007 Sep 26.
Abstract
We analyzed the levels of acetylated histones and histone H3 dimethylated on lysine 4 (H3K4me2) at the LMP2A promoter (LMP2Ap) of Epstein-Barr virus in well-characterized type I and type III lymphoid cell line pairs and additionally in the nasopharyngeal carcinoma cell line C666-1 by using chromatin immunoprecipitation. We found that enhanced levels of acetylated histones marked the upregulated LMP2Ap in lymphoid cells. In contrast, in C666-1 cells, the highly DNA-methylated, inactive LMP2Ap was also enriched in acetylated histones and H3K4me2. Our results suggest that the combinatorial effects of DNA methylation, histone acetylation, and H3K4me2 modulate the activity of LMP2Ap.
摘要
我们通过染色质免疫沉淀法，分析了爱泼斯坦-巴尔病毒潜伏膜蛋白2A启动子（LMP2Ap）上乙酰化组蛋白以及赖氨酸4二甲基化组蛋白H3（H3K4me2）的水平，这些分析是在特征明确的I型和III型淋巴样细胞系对中进行的，另外还在鼻咽癌细胞系C666-1中进行了分析。我们发现，乙酰化组蛋白水平的升高标志着淋巴样细胞中LMP2Ap的上调。相比之下，在C666-1细胞中，高度DNA甲基化的无活性LMP2Ap也富含乙酰化组蛋白和H3K4me2。我们的结果表明，DNA甲基化、组蛋白乙酰化和H3K4me2的联合作用调节了LMP2Ap的活性。
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