转录型和非转录型劳氏肉瘤前病毒细胞整合位点之间的差异。
Differences between cellular integration sites of transcribed and nontranscribed Rous sarcoma proviruses.
作者信息
Fincham V J, Wyke J A
机构信息
Beatson Institute for Cancer Research, Cancer Research Campaign Beatson Laboratories, United Kingdom.
出版信息
J Virol. 1991 Jan;65(1):461-3. doi: 10.1128/JVI.65.1.461-463.1991.
Abstract
Transcribed Rous sarcoma proviruses in Rat-1 DNA tend to integrate closely 3' to C-G-rich restriction enzyme sites, and 2 of 13 such proviruses were found to have inserted at the same locus. However, most integrated proviruses were transcriptionally silent insertions at sites indistinguishable from random. We conclude that Rous sarcoma proviruses in rat cells usually fail to integrate in a favorable site for transcription, in contrast to studies on proviral integration in permissive hosts.
摘要
转录到大鼠-1细胞DNA中的劳氏肉瘤前病毒倾向于紧密整合到富含C-G的限制性酶切位点的3'端，并且在13个这样的前病毒中，有2个被发现插入到了同一基因座。然而，大多数整合的前病毒是在与随机位点难以区分的位点上进行转录沉默插入。我们得出结论，与在允许性宿主中进行的前病毒整合研究相反，大鼠细胞中的劳氏肉瘤前病毒通常无法整合到有利于转录的位点。
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