Genome. 1993 Apr;36(2):216-23. doi: 10.1139/g93-030.
Nuclear restriction fragment length polymorphisms (RFLPs) were used to determine phylogenetic relationships in the genus Cajanus using 15 random genomic probes and six restriction enzymes. Twenty-four accessions representing 12 species of four genera (Cajanus, Dunbaria, Eriosema, and Rhynchosia) were examined to determine phylogenetic relationships in the genus Cajanus. Eriosema parviflorum was selected as the out-group. Sufficient RFLP polymorphisms were detected among species to resolve in-group taxa into distinct clusters. Topologies of trees from parsimony and similarity matrix analyses were similar but not identical, and clustering patterns agreed broadly with published phylogenies based on seed protein data and, to a lesser extent, data from cytology and breeding experiments. Accessions of cultivated C. cajan shared more DNA fragments with C. scarabaeoides than with C. cajanifolia. Inconsistencies in taxonomic relationships based on data from morphology, cytology, crossability, and RFLPs are discussed.
使用 15 个随机基因组探针和 6 种限制酶，对 12 种 4 属（Cajanus、Dunbaria、Eriosema 和 Rhynchosia）的 24 个材料进行核限制片段长度多态性（RFLP）分析，以确定 Cajanus 属的系统发育关系。选择 Eriosema parviflorum 作为外类群。在种间检测到足够的 RFLP 多态性，可将内类群分类群分为不同的聚类。简约和相似矩阵分析的树拓扑结构相似但不完全相同，聚类模式与基于种子蛋白数据的发表的系统发育以及在较小程度上基于细胞学和育种实验数据的系统发育基本一致。栽培 C. cajan 的材料与 C. scarabaeoides 共享的 DNA 片段多于 C. cajanifolia。讨论了基于形态学、细胞学、可交配性和 RFLP 数据的分类关系中的不一致性。
