Singh A K, Rai V P, Chand R, Singh R P, Singh M N
Department of Genetics and Plant Breeding, Institute of Agricultural Sciences, Banaras Hindu University, Varanas 221 005, India.
J Genet. 2013;92(2):273-80. doi: 10.1007/s12041-013-0266-7.
Genetic diversity and identification of simple sequence repeat markers correlated with Fusarium wilt resistance was performed in a set of 36 elite cultivated pigeonpea genotypes differing in levels of resistance to Fusarium wilt. Twenty-four polymorphic sequence repeat markers were screened across these genotypes, and amplified a total of 59 alleles with an average high polymorphic information content value of 0.52. Cluster analysis, done by UPGMA and PCA, grouped the 36 pigeonpea genotypes into two main clusters according to their Fusarium wilt reaction. Based on the Kruskal-Wallis ANOVA and simple regression analysis, six simple sequence repeat markers were found to be significantly associated with Fusarium wilt resistance. The phenotypic variation explained by these markers ranged from 23.7 to 56.4%. The present study helps in finding out feasibility of prescreened SSR markers to be used in genetic diversity analysis and their potential association with disease resistance.
在一组36个对枯萎病抗性水平不同的优良栽培木豆基因型中，进行了与枯萎病抗性相关的遗传多样性分析和简单序列重复标记鉴定。在这些基因型中筛选了24个多态性序列重复标记，共扩增出59个等位基因，平均高多态信息含量值为0.52。通过非加权组平均法（UPGMA）和主成分分析（PCA）进行聚类分析，根据木豆对枯萎病的反应，将36个木豆基因型分为两个主要类群。基于Kruskal-Wallis方差分析和简单回归分析，发现6个简单序列重复标记与枯萎病抗性显著相关。这些标记解释的表型变异范围为23.7%至56.4%。本研究有助于了解预筛选的SSR标记用于遗传多样性分析的可行性及其与抗病性的潜在关联。
