Genome. 1995 Apr;38(2):406-13. doi: 10.1139/g95-052.
Wheatgrasses (species of Agropyron complex) have previously been reported to be resistant to barley yellow dwarf virus (BYDV). To introgress this resistance into wheat, Triticum aestivum x Thinopyrum (Agropyron) intermedium hybrids were advanced through a backcrossing program and reaction to BYDV, as determined by enzyme-linked immunosorbent assay (ELISA), is reported for the first time in backcross populations of wide hybrids between wheat and wheatgrasses. ELISA values revealed highly resistant to highly susceptible segregants in backcrosses. BYDV resistance was expressed in some backcross derivatives. Continued selection, based on cytology and ELISA in each generation, eliminated most of the unwanted wheatgrass chromosomes and produced self-fertile BYDV resistant wheat lines. The BYDV resistant lines with 2n = 42 had normal chromosome pairing similar to wheat, and their F1 hybrids with wheat had two univalents. DNA analyses showed that the source of alien chromatin in these BYDV resistant wheat lines is distinguishable from that in other Th. intermedium derived BYDV resistant wheat lines. Chromosome pairing and restriction fragment length polymorphism analyses indicated that the 42 chromosome resistant Purdue wheat lines are substitution lines in which chromosome 7D was replaced by a chromosome from Th. intermedium that was carrying gene(s) for BYDV resistance.
小麦草（Agropyron 复合体的物种）以前被报道对大麦黄花叶病毒（BYDV）具有抗性。为了将这种抗性导入小麦，通过回交计划和酶联免疫吸附测定（ELISA）来确定对 BYDV 的反应，将 Triticum aestivum x Thinopyrum（Agropyron）中间杂种进行了推进，这是首次在小麦和小麦草之间的宽杂交回交群体中报告。ELISA 值显示回交中高度抗性到高度敏感的分离物。在一些回交衍生品种中表达了 BYDV 抗性。基于细胞学和每代的 ELISA 进行持续选择，消除了大多数不需要的小麦草染色体，并产生了自交可育的 BYDV 抗性小麦系。具有 2n = 42 的 BYDV 抗性系与小麦相似，具有正常的染色体配对，并且它们与小麦的 F1 杂种具有两个单价体。DNA 分析表明，这些 BYDV 抗性小麦系中外源染色质的来源与其他源自 Th. intermedium 的 BYDV 抗性小麦系中的来源不同。染色体配对和限制性片段长度多态性分析表明，42 条染色体的抗性普度小麦系是替代系，其中 7D 染色体被携带 BYDV 抗性基因的 Th. intermedium 染色体所取代。
