使用单克隆抗体通过属特异性抗原捕获酶免疫测定法检测甲病毒。
Detection of alphaviruses in a genus-specific antigen capture enzyme immunoassay using monoclonal antibodies.
作者信息
Greiser-Wilke I M, Moennig V, Kaaden O R, Shope R E
机构信息
Institute of Virology, Hannover Veterinary School, Federal Republic of Germany.
出版信息
J Clin Microbiol. 1991 Jan;29(1):131-7. doi: 10.1128/jcm.29.1.131-137.1991.
Abstract
A genus-specific antigen capture assay using similar combinations of monoclonal antibodies for capture and detection of 24 alphaviruses belonging to the seven serocomplexes was developed. The sensitivity of the test ranged from 10(3.4) 50% tissue culture infective doses/ml for o'nyong-nyong virus to 10(6.1) 50% tissue culture infective doses/ml for Middelburg virus. The antigen capture test uses a combination of cross-reacting monoclonal antibodies directed against the nucleocapsid protein and envelope glycoprotein E1 of Semliki Forest virus.
摘要
开发了一种属特异性抗原捕获测定法，该方法使用相似组合的单克隆抗体来捕获和检测属于七个血清复合体的24种甲病毒。该检测的灵敏度范围为：奥尼昂-尼昂病毒为10(3.4) 50%组织培养感染剂量/毫升，而米德尔堡病毒为10(6.1) 50%组织培养感染剂量/毫升。抗原捕获试验使用了针对塞姆利基森林病毒核衣壳蛋白和包膜糖蛋白E1的交叉反应单克隆抗体组合。
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