类风湿关节炎和系统性红斑狼疮患者循环中MMP - 2和MMP - 9水平及活性升高。

Elevated circulatory MMP-2 and MMP-9 levels and activities in patients with rheumatoid arthritis and systemic lupus erythematosus.

作者信息

Chang Yih-Hsin, Lin I-Ling, Tsay Gregory J, Yang Shun-Chun, Yang Tzi-Peng, Ho Kuo-Ting, Hsu Tsai-Ching, Shiau Ming-Yuh

机构信息

School of Medical Laboratory and Biotechnology, Chung Shan Medical University, Taichung, Taiwan, Republic of China.

出版信息

Clin Biochem. 2008 Aug;41(12):955-9. doi: 10.1016/j.clinbiochem.2008.04.012. Epub 2008 Apr 26.

DOI:10.1016/j.clinbiochem.2008.04.012

PMID:18471998

Abstract

OBJECTIVES

Matrix metalloproteinases (MMPs) are suggested to play important roles in rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). This study is to examine the MMPs expressions and activities in Taiwanese RA and SLE patients.

DESIGN AND METHODS

Levels and activities of plasma MMP-2 and MMP-9 were investigated by enzyme-linked immunosorbent assay and zymography, respectively.

RESULTS

MMP-2 levels in control subjects, RA and SLE patients were 146.1+/-34.2, 194.0+/-24.2 and 208.9+/-75.9 ng/mL respectively, and for MMP-9 were 51.4+/-57.1, 567.7+/-313.1 and 208.7+/-105.5 ng/mL respectively. Both MMP-2 and MMP-9 levels and activities from all patients were significantly higher than that from control subjects.

CONCLUSIONS

MMP-2 levels in both patients groups were approximately 1.3-1.4 folds higher than that in control subjects, notably, MMP-9 levels were 11- and 4-folds significantly higher, respectively, in RA and SLE patients. The results which MMP-2 and MMP-9 levels and activities are significantly elevated support the involvement of MMPs proteins in these autoimmune disorders.

摘要

目的

基质金属蛋白酶（MMPs）被认为在类风湿关节炎（RA）和系统性红斑狼疮（SLE）中起重要作用。本研究旨在检测台湾地区RA和SLE患者体内MMPs的表达及活性。

设计与方法

分别采用酶联免疫吸附测定法和酶谱分析法检测血浆中MMP - 2和MMP - 9的水平及活性。

结果

对照组、RA患者和SLE患者的MMP - 2水平分别为146.1±34.2、194.0±24.2和208.9±75.9 ng/mL，MMP - 9水平分别为51.4±57.1、567.7±313.1和208.7±105.5 ng/mL。所有患者的MMP - 2和MMP - 9水平及活性均显著高于对照组。