Hanke T, Graham F L, Rosenthal K L, Johnson D C
Department of Pathology, McMaster University, Hamilton, Ontario, Canada.
J Virol. 1991 Mar;65(3):1177-86. doi: 10.1128/JVI.65.3.1177-1186.1991.
Cytotoxic T-lymphocyte (CTL) responses to herpes simplex virus (HSV) polypeptides play an important role in recovery from infection and in preventing latency. We have previously shown that glycoprotein B (gB) is a major target recognized by HSV-specific CTLs in C57BL/6 (H-2b) and BALB/c (H-2d) mice but not in CBA/J (H-2k) mice (L. A. Witmer, K. L. Rosenthal, F. L. Graham, H. M. Friedman, A. Yee, and D. C. Johnson, J. Gen. Virol. 71:387-396, 1990). In this report, we utilize adenovirus vectors expressing gB with various deletions to localize an immunodominant site in gB, recognized by H-2b-restricted anti-HSV CTLs, to a region between residues 462 and 594. Overlapping peptides spanning this region were synthesized and used to further localize the immunodominant site to residues 489 to 515, a region highly conserved in HSV type 1 (HSV-1) and HSV-2 strains. The 11-amino-acid peptide was apparently associated exclusively with the Kb major histocompatibility complex gene product and not the Db gene product. In contrast, H-2d-restricted CTLs recognized an immunodominant site between residues 233 and 379.
细胞毒性T淋巴细胞（CTL）对单纯疱疹病毒（HSV）多肽的反应在感染恢复和预防潜伏中起重要作用。我们之前已经表明，糖蛋白B（gB）是C57BL/6（H-2b）和BALB/c（H-2d）小鼠中HSV特异性CTL识别的主要靶标，但在CBA/J（H-2k）小鼠中不是（L.A. Witmer、K.L. Rosenthal、F.L. Graham、H.M. Friedman、A. Yee和D.C. Johnson，《病毒学杂志》71:387 - 396，1990年）。在本报告中，我们利用表达具有各种缺失的gB的腺病毒载体，将H-2b限制性抗HSV CTL识别的gB中的免疫显性位点定位到462至594位残基之间的区域。合成了跨越该区域的重叠肽，并用于将免疫显性位点进一步定位到489至515位残基，这是1型单纯疱疹病毒（HSV-1）和2型单纯疱疹病毒（HSV-2）毒株中高度保守的区域。这个11个氨基酸的肽显然仅与Kb主要组织相容性复合体基因产物相关，而与Db基因产物无关。相比之下，H-2d限制性CTL识别233至379位残基之间的一个免疫显性位点。
