应用随机扩增多态性DNA指纹技术分析A群链球菌引起的产褥热暴发
Analysis of an outbreak of puerperal Fever due to group a streptococci by random amplified polymorphic DNA fingerprinting.
作者信息
Meis J F, Muytjens H L, van den Berg P P, Voss A, Melchers W J
机构信息
Department of Medical Microbiology MMB 440 P.O. Box 9101 University Hospital Nijmegen Nijmegen 6500 HB The Netherlands.
出版信息
Infect Dis Obstet Gynecol. 1997;5(3):232-6. doi: 10.1155/S1064744997000392.
OBJECTIVE
Streptococcus pyogenes is the cause of the classical childbed fever and can occur in both sporadic and epidemic form. Once an outbreak is identified on a maternity ward it is not only necessary to place the patients in strict isolation but also identify to the source of the infection. Fast reliable typing methods can aid in infection control.
METHODS
An outbreak of puerperal fever due to S. pyogenes was analyzed by random amplified polymorphic DNA (RAPD) analysis.
RESULTS
Identical fingerprint patterns were found in isolates of 3 patients, the throat and infected finger of the delivering obstetrician, 2 of the physician's family members, and from the cervix of a woman who was examined by the physician 7 months after the outbreak. The outbreak was stopped after antimicrobial treatment of the physician and his family members.
CONCLUSIONS
RAPD typing appeared to be a fast and reliable tool for epidemiological studies of S. pyogenes and is probably more efficient in strain differentiation than classical M and T serotyping.
目的
化脓性链球菌是典型产褥热的病因，可呈散发性和流行性。一旦在产科病房发现疫情，不仅要对患者进行严格隔离，还要确定感染源。快速可靠的分型方法有助于感染控制。
方法
采用随机扩增多态性DNA（RAPD）分析对一起由化脓性链球菌引起的产褥热疫情进行分析。
结果
在3名患者的分离株、接生产科医生的咽喉部和感染手指、医生的2名家庭成员以及疫情爆发7个月后由该医生检查的一名女性的宫颈中发现了相同的指纹图谱。在对医生及其家庭成员进行抗菌治疗后，疫情得到控制。
结论
RAPD分型似乎是化脓性链球菌流行病学研究的一种快速可靠工具，在菌株鉴别方面可能比传统的M和T血清分型更有效。
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