比较验证研究，以证明对AOAC方法996.09[大肠杆菌O157:H7的可视化免疫沉淀法（VIP）]进行的微小修改与参考培养方法等效。

Comparative validation study to demonstrate the equivalence of a minor modification to AOAC Method 996.09 [Visual Immunoprecipitate (VIP) for E. coli O157:H7] to the reference culture methods.

作者信息

Feldsine Philip T, Kerr David E, Shen George S, Lienau Andrew H

机构信息

BioControl Systems, Inc., 12822 SE 32nd St, Bellevue, WA 98005, USA.

出版信息

J AOAC Int. 2008 Mar-Apr;91(2):360-4.

Abstract

The Visual Immunoprecipitate (VIP) for the Detection of E. coli O157:H7 in Foods, AOAC Official Method 996.09, has been modified to use a simplified plastic housing for the device. A methods comparison study was conducted to demonstrate the equivalence of this modification to the reference culture method. Three foods were analyzed. In total, valid results were obtained from 240 samples and controls. Results showed that the VIP for E. coli O157:H7 is equivalent to the reference culture methods for the detection of E. coli O157:H7.

摘要

用于检测食品中大肠杆菌O157:H7的视觉免疫沉淀物（VIP），AOAC官方方法996.09，已进行修改，采用简化的塑料外壳装置。开展了一项方法对比研究，以证明此修改版本与参考培养方法等效。对三种食品进行了分析。总共从240个样品和对照中获得了有效结果。结果表明，用于检测大肠杆菌O157:H7的VIP与检测大肠杆菌O157:H7的参考培养方法等效。

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