Sakagami H, Kawazoe Y, Oh-hara T, Kitajima K, Inoue Y, Tanuma S, Ichikawa S, Konno K
First Department of Biochemistry, School of Medicine, Showa University, Japan.
J Leukoc Biol. 1991 Mar;49(3):277-82. doi: 10.1002/jlb.49.3.277.
Lignin is a heterogenous natural product composed of phenylpropane units and is usually associated with hemicellulose in its native state. Until now little attention has been paid to the potential therapeutic utility of lignified products. Natural lignified products are demonstrated in the present study to stimulate iodination significantly (incorporation of radioactive iodine into an acid-insoluble fraction) of human peripheral blood polymorphonuclear cells (PMN). This stimulation was significantly inhibited in the presence of myeloperoxidase inhibitors. These materials were almost completely deprived of their stimulation capacity by treatment with NaCIO2, but this capacity was not affected by severe treatment with H2SO4 or trifluoroacetic acid. Similar stimulating activity by chemically defined tannin-related polyphenolic compounds was observed. Degradation products or component units of lignin, and natural antitumor polysaccharides and their chemically modified derivatives (introduced with negatively or positively charged groups) and polysialoglycoproteins had little or no activity. The results indicate the importance of a polymerized phenolic structure for the stimulation of PMN iodination. Possible physiological relevance of the stimulation of iodination by lignified substances is discussed.
木质素是一种由苯丙烷单元组成的异质天然产物，在其天然状态下通常与半纤维素结合。到目前为止，木质化产物的潜在治疗用途很少受到关注。本研究表明，天然木质化产物能显著刺激人外周血多形核细胞（PMN）的碘化作用（将放射性碘掺入酸不溶性部分）。在髓过氧化物酶抑制剂存在的情况下，这种刺激作用受到显著抑制。用NaClO₂处理后，这些物质的刺激能力几乎完全丧失，但用H₂SO₄或三氟乙酸进行严格处理并不影响其刺激能力。观察到化学定义的单宁相关多酚化合物具有类似的刺激活性。木质素的降解产物或组成单元、天然抗肿瘤多糖及其化学修饰衍生物（引入带负电荷或正电荷的基团）以及多唾液酸糖蛋白几乎没有或没有活性。结果表明聚合酚结构对刺激PMN碘化的重要性。讨论了木质化物质刺激碘化作用可能的生理相关性。
