Department of Parasitology and Brain Korea 21 for Medical Science, Hanyang University College of Medicine, Seoul, Korea.
Parasite Immunol. 2008 Aug;30(8):410-6. doi: 10.1111/j.1365-3024.2008.01037.x.
Neutrophils are the predominant inflammatory cells found in the vaginal discharge of patients with a Trichomonas vaginalis infection. Neutrophils have a shorter life span than other leucocytes. Our previous study indicated that live T. vaginalis alters Mcl-1 expression and caspase-3 activation, thereby inducing apoptosis of human neutrophils. However, it was previously unknown that the apoptotic neutrophils brought about by T. vaginalis can influence vaginal inflammation. Thus, human monocyte-derived macrophages (HMDM) were incubated with T. vaginalis-induced apoptotic neutrophils. Cytokine production and phagocytosis by HMDM were evaluated by ELISA and myeloperoxidase stain, respectively. HMDM showed increased anti-inflammatory cytokine production (IL-10) and decreased levels of pro-inflammatory cytokines, such as TNF-α and IL-6, compared with macrophages alone.
中性粒细胞是滴虫感染患者阴道分泌物中占主导地位的炎性细胞。中性粒细胞的寿命比其他白细胞短。我们之前的研究表明，活滴虫改变了 Mcl-1 的表达和 caspase-3 的激活，从而诱导人中性粒细胞凋亡。然而，之前尚不清楚滴虫引起的凋亡中性粒细胞会影响阴道炎症。因此，我们用人单核细胞来源的巨噬细胞（HMDM）孵育了由滴虫诱导的凋亡中性粒细胞。通过 ELISA 和髓过氧化物酶染色分别评估 HMDM 的细胞因子产生和吞噬作用。与单独的巨噬细胞相比，HMDM 显示出抗炎细胞因子（IL-10）产生增加，促炎细胞因子（如 TNF-α 和 IL-6）水平降低。
