蛋白质可塑性：酿酒酵母氧化角鲨烯-羊毛甾醇环化酶中的单个氨基酸取代产生原托-13(17),24-二烯-3β-醇，一种重排产物。

Protein plasticity: a single amino acid substitution in the Saccharomyces cerevisiae oxidosqualene-lanosterol cyclase generates protosta-13(17),24-dien-3beta-ol, a rearrangement product.

作者信息

Wu Tung-Kung, Wen Hao-Yu, Chang Cheng-Hsiang, Liu Yuan-Ting

机构信息

Department of Biological Science and Technology, Institute of Molecular Science, National Chiao Tung University, Hsin-Chu, Taiwan, ROC.

出版信息

Org Lett. 2008 Jun 19;10(12):2529-32. doi: 10.1021/ol800799n.

DOI:10.1021/ol800799n

PMID:18494476

Abstract

To provide insights into the structure-function relationships of oxidosqualene-lanosterol cyclase (ERG7) from Saccharomyces cerevisiae, the Phe699 was exchanged against hydrophilic polar uncharged residues Ser, Thr, Cys, Gln, and Tyr to characterize its product profile and functional role in ERG7 activity. Among the substitutions, only the ERG7(F699T) mutant produced novel protosta-13(17),24-dien-3beta-ol as the sole truncated rearrangement product. The results suggest that Phe699Thr mutation is likely to affect the C-17 cation stabilization during the rearrangement process.

摘要

为深入了解酿酒酵母氧化角鲨烯-羊毛甾醇环化酶（ERG7）的结构-功能关系，将苯丙氨酸699替换为亲水性极性不带电残基丝氨酸、苏氨酸、半胱氨酸、谷氨酰胺和酪氨酸，以表征其产物谱及其在ERG7活性中的功能作用。在这些替换中，只有ERG7（F699T）突变体产生了新型原甾-13（17），24-二烯-3β-醇作为唯一的截短重排产物。结果表明，苯丙氨酸699到苏氨酸的突变可能会影响重排过程中C-17阳离子的稳定性。