López-López G, Pascual A, Martínez-Martínez L, Perea E J
Department of Microbiology, School of Medicine, University of Seville, Spain.
Diagn Microbiol Infect Dis. 1991 Jan-Feb;14(1):1-6. doi: 10.1016/0732-8893(91)90078-t.
The effect of a siliconized latex urinary catheter on the in vitro adherence and growth of Pseudomonas aeruginosa and Escherichia coli and on the activity of human polymorphonuclear leukocytes (PMNs) was assessed. The adherence of P. aeruginosa to latex catheters was significantly greater than that of E. coli, being 30 times higher at 6 hr of incubation. The survival of E. coli (10(8) CFU/ml) in phosphate-buffered saline (PBS) containing segments of siliconized latex catheters was lower than in the controls. This effect seems to be due to a toxic effect produced by substances eluted into the medium from the catheters, as the viability of E. coli (10(3) CFU/ml) in eluates obtained from the incubation of catheters in PBS (24 hr; 37 degrees C) was significantly lower than in the controls. These phenomena were not observed with P. aeruginosa. The incubation of human PMNs with either catheter segments or eluates did not affect the uptake of opsonized E. coli but significantly decreased the production of superoxide radicals by these phagocytes. It is concluded that the adherence of P. aeruginosa to these catheters is higher than that of E. coli and that the latex urinary catheter used herein elutes substances that are in vitro toxic for E. coli and the oxygen-dependent bactericidal mechanisms of human PMNs.
评估了硅化乳胶导尿管对铜绿假单胞菌和大肠杆菌的体外黏附及生长以及对人多形核白细胞（PMN）活性的影响。铜绿假单胞菌对乳胶导尿管的黏附显著高于大肠杆菌，在孵育6小时时高出30倍。在含有硅化乳胶导尿管片段的磷酸盐缓冲盐水（PBS）中，大肠杆菌（10⁸CFU/ml）的存活率低于对照组。这种效应似乎是由于从导管洗脱到培养基中的物质产生的毒性作用，因为在PBS（24小时；37℃）中孵育导管获得的洗脱液中大肠杆菌（10³CFU/ml）的活力显著低于对照组。铜绿假单胞菌未观察到这些现象。将人PMN与导管片段或洗脱液一起孵育不影响调理素化大肠杆菌的摄取，但显著降低了这些吞噬细胞超氧自由基的产生。得出的结论是，铜绿假单胞菌对这些导管的黏附高于大肠杆菌，并且本文使用的乳胶导尿管洗脱的物质在体外对大肠杆菌和人PMN的氧依赖性杀菌机制有毒性。
