Berthier Erwin, Warrick Jay, Yu Hongmeiy, Beebe David J
MacDiarmid Institute for Advanced Materials and Nanotechnology, Department of Electrical and Computer Engineering, University of Canterbury, Christchurch, NZ.
Lab Chip. 2008 Jun;8(6):852-9. doi: 10.1039/b717422e. Epub 2008 Apr 8.
Evaporation is a well known issue when handling small liquid volumes. Here we present a review of microscale assays prone to evaporation and methods to make them more robust. Applications for these assays span from combinatorial chemistry to cell-biology where the stability of concentrations and osmolarity can be critical. A dimensionless evaporation number Ev is presented and used to characterize volume loss in short term and long term microscale assays. Ev can be used both as a design tool and as an analysis parameter. The advantageous use of evaporation in some applications is also discussed.
在处理少量液体时，蒸发是一个众所周知的问题。在此，我们综述了易于蒸发的微尺度分析方法以及增强其稳定性的方法。这些分析方法的应用范围涵盖从组合化学到细胞生物学等领域，在这些领域中，浓度和渗透压的稳定性可能至关重要。我们提出了一个无量纲蒸发数Ev，并用于表征短期和长期微尺度分析中的体积损失。Ev既可以用作设计工具，也可以用作分析参数。我们还讨论了蒸发在某些应用中的有益用途。
