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对血清中的核酸外切酶降解具有稳定性的修饰脱氧寡核苷酸。

Modified deoxyoligonucleotides stable to exonuclease degradation in serum.

作者信息

Shaw J P, Kent K, Bird J, Fishback J, Froehler B

机构信息

Gilead Sciences, Foster City, CA 94404.

出版信息

Nucleic Acids Res. 1991 Feb 25;19(4):747-50. doi: 10.1093/nar/19.4.747.

DOI:10.1093/nar/19.4.747
PMID:1850122
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC333706/
Abstract

Unmodified deoxyoligonucleotides are rapidly degraded in serum-containing medium. Utilizing internally labelled deoxyoligonucleotides the deoxyribonuclease profile for fetal calf serum and human serum was determined. It was found that the predominate nuclease activity in both systems was 3' exonuclease. Deoxyoligonucleotides are protected from exonuclease degradation in sera and cell media by simple terminal modifications that maintain high binding activity for the complementary DNA sequence.

摘要

未修饰的脱氧寡核苷酸在含血清的培养基中会迅速降解。利用内部标记的脱氧寡核苷酸测定了胎牛血清和人血清的脱氧核糖核酸酶谱。结果发现，两个体系中主要的核酸酶活性都是3'外切核酸酶。通过简单的末端修饰可保护脱氧寡核苷酸在血清和细胞培养基中免受外切核酸酶降解，这些修饰能保持对互补DNA序列的高结合活性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d14/333706/70d79456a5d1/nar00240-0058-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d14/333706/98f16dcf4a8b/nar00240-0057-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d14/333706/70d79456a5d1/nar00240-0058-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d14/333706/98f16dcf4a8b/nar00240-0057-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d14/333706/70d79456a5d1/nar00240-0058-a.jpg

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