Mandalari G, Nueno-Palop C, Bisignano G, Wickham M S J, Narbad A
The Model Gut Platform, Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, United Kingdom.
Appl Environ Microbiol. 2008 Jul;74(14):4264-70. doi: 10.1128/AEM.00739-08. Epub 2008 May 23.
Almonds are known to have a number of nutritional benefits, including cholesterol-lowering effects and protection against diabetes. They are also a good source of minerals and vitamin E, associated with promoting health and reducing the risk for chronic disease. For this study we investigated the potential prebiotic effect of almond seeds in vitro by using mixed fecal bacterial cultures. Two almond products, finely ground almonds (FG) and defatted finely ground almonds (DG), were subjected to a combined model of the gastrointestinal tract which included in vitro gastric and duodenal digestion, and the resulting fractions were subsequently used as substrates for the colonic model to assess their influence on the composition and metabolic activity of gut bacteria populations. FG significantly increased the populations of bifidobacteria and Eubacterium rectale, resulting in a higher prebiotic index (4.43) than was found for the commercial prebiotic fructooligosaccharides (4.08) at 24 h of incubation. No significant differences in the proportions of gut bacteria groups were detected in response to DG. The increase in the numbers of Eubacterium rectale during fermentation of FG correlated with increased butyrate production. In conclusion, we have shown that the addition of FG altered the composition of gut bacteria by stimulating the growth of bifidobacteria and Eubacterium rectale.
杏仁具有多种营养益处，包括降低胆固醇的作用以及预防糖尿病。它们也是矿物质和维生素E的良好来源，与促进健康和降低慢性病风险相关。在本研究中，我们通过使用混合粪便细菌培养物，在体外研究了杏仁种子的潜在益生元效应。两种杏仁产品，细磨杏仁（FG）和脱脂细磨杏仁（DG），经过包括体外胃和十二指肠消化的胃肠道联合模型处理，所得部分随后用作结肠模型的底物，以评估它们对肠道细菌群体组成和代谢活性的影响。FG显著增加了双歧杆菌和直肠真杆菌的数量，在孵育24小时时产生了比商业益生元低聚果糖（4.08）更高的益生元指数（4.43）。未检测到DG处理后肠道细菌组比例的显著差异。FG发酵过程中直肠真杆菌数量的增加与丁酸盐产量的增加相关。总之，我们已经表明，添加FG通过刺激双歧杆菌和直肠真杆菌的生长改变了肠道细菌的组成。
