Quéméneur Marianne, Heinrich-Salmeron Audrey, Muller Daniel, Lièvremont Didier, Jauzein Michel, Bertin Philippe N, Garrido Francis, Joulian Catherine
BRGM, Ecotechnology Unit, Process and Environment Division, 3 Avenue Claude Guillemin, 45060 Orléans Cedex 02, France.
Appl Environ Microbiol. 2008 Jul;74(14):4567-73. doi: 10.1128/AEM.02851-07. Epub 2008 May 23.
A new primer set was designed to specifically amplify ca. 1,100 bp of aoxB genes encoding the As(III) oxidase catalytic subunit from taxonomically diverse aerobic As(III)-oxidizing bacteria. Comparative analysis of AoxB protein sequences showed variable conservation levels and highlighted the conservation of essential amino acids and structural motifs. AoxB phylogeny of pure strains showed well-discriminated taxonomic groups and was similar to 16S rRNA phylogeny. Alphaproteobacteria-, Betaproteobacteria-, and Gammaproteobacteria-related sequences were retrieved from environmental surveys, demonstrating their prevalence in mesophilic As-contaminated soils. Our study underlines the usefulness of the aoxB gene as a functional marker of aerobic As(III) oxidizers.
设计了一套新的引物，用于特异性扩增来自不同分类群的好氧性砷（III）氧化细菌中编码砷（III）氧化酶催化亚基的aoxB基因的约1100 bp片段。对AoxB蛋白序列的比较分析显示出不同的保守水平，并突出了必需氨基酸和结构基序的保守性。纯菌株的AoxB系统发育显示出分类群的良好区分，并且与16S rRNA系统发育相似。从环境调查中检索到了与α-变形菌纲、β-变形菌纲和γ-变形菌纲相关的序列，证明它们在中温砷污染土壤中普遍存在。我们的研究强调了aoxB基因作为好氧性砷（III）氧化菌功能标记的有用性。
