Martínez-Aguilar Lourdes, Díaz Rafael, Peña-Cabriales Juan José, Estrada-de Los Santos Paulina, Dunn Michael F, Caballero-Mellado Jesús
Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Ap. Postal 565-A, Cuernavaca, Morelos, México.
Appl Environ Microbiol. 2008 Jul;74(14):4574-9. doi: 10.1128/AEM.00201-08. Epub 2008 May 23.
Pulsed-field gel electrophoresis and 16S rRNA hybridization experiments showed that multichromosome genome structures and very large genome sizes (6.46 to 8.73 Mb) are prevalent in novel plant-associated Burkholderia species. (15)N(2) isotope dilution assays revealed unambiguous diazotrophy in these novel species. nifH gene sequence analysis, often used to determine phylogenetic relatedness among diazotrophs, showed tight clusters of Burkholderia species, which were clearly distinct from those of other diazotrophs.
脉冲场凝胶电泳和16S rRNA杂交实验表明,多染色体基因组结构和非常大的基因组大小(6.46至8.73 Mb)在新发现的与植物相关的伯克霍尔德氏菌属物种中很普遍。(15)N(2)同位素稀释试验表明这些新物种中存在明确的固氮作用。常用于确定固氮菌之间系统发育相关性的nifH基因序列分析显示,伯克霍尔德氏菌属物种紧密聚类,明显不同于其他固氮菌。