Lédée N, Lombroso R, Lombardelli L, Selva J, Dubanchet S, Chaouat G, Frankenne F, Foidart J M, Maggi E, Romagnani S, Ville Y, Piccinni M-P
Service de Gynécologie-Obstétrique et Médecine de la Reproduction et Service de cytogenétique et Biologie de la Reproduction, Centre hospitalier intercommunal Poissy-Saint Germain en Laye, Univ UVSQ, UPRES-EA 2493, Poissy, France.
Hum Reprod. 2008 Sep;23(9):2001-9. doi: 10.1093/humrep/den192. Epub 2008 May 24.
The cytokine/chemokine levels of individual follicular fluids (FFs) were measured to determine whether a biomarker could be linked to the developmental potential of the derived embryo.
Fluid was collected from 132 individual FFs that were the source of oocytes subsequently fertilized and transferred. In each, a bead-based multiplex sandwich immunoassay (Luminex) was used to measure 28 cytokines and chemokines simultaneously.
Significantly higher levels of interleukin (IL-2) and interferon (IFN-gamma) were detected in FF for embryos that underwent early cleavage. IL-12 was significantly higher in FF corresponding to highly fragmented embryos and the chemokine CCL5 was significantly higher in FF related to the best quality (Top) embryos. The level of granulocyte colony-stimulating factor (G-CSF) in individual FF samples was correlated with the implantation potential of the corresponding embryo. The area under the receiver operating characteristics curve, which distinguished the embryos that definitely led to delivery from those that did not, was 0.84 (0.75-0.90) (P = 0.0001) for FF G-CSF. FF G-CSF was significantly lower in patients older than 36 years compared with those <30-year old. When the FF G-CSF was 20 pg/ml or higher, the ratio between Top and non-Top embryos was significantly higher than for the group with FF G-CSF below 20 pg/ml (45 versus 20.45%, P = 0.007).
Individual FF composition is related to the development of the corresponding in vitro generated embryo and its potential of implantation. Individual FF G-CSF may provide a non-invasive biomarker of implantation that needs to be evaluated together with in vitro observation to select the oocyte, and hence the embryo, to transfer.
测量单个卵泡液（FF）中的细胞因子/趋化因子水平，以确定是否存在与衍生胚胎发育潜力相关的生物标志物。
从132个单个卵泡液中收集液体，这些卵泡液是随后受精并移植的卵母细胞的来源。在每个卵泡液中，使用基于微珠的多重夹心免疫测定法（Luminex）同时测量28种细胞因子和趋化因子。
在早期分裂的胚胎的卵泡液中检测到白细胞介素（IL-2）和干扰素（IFN-γ）水平显著更高。在高度碎片化胚胎对应的卵泡液中，IL-12显著更高，趋化因子CCL5在与最佳质量（顶级）胚胎相关的卵泡液中显著更高。单个卵泡液样本中的粒细胞集落刺激因子（G-CSF）水平与相应胚胎的着床潜力相关。区分肯定能导致分娩的胚胎与不能导致分娩的胚胎的受试者工作特征曲线下面积，对于卵泡液G-CSF为0.84（0.75 - 0.90）（P = 0.0001）。与30岁以下的患者相比，36岁以上患者的卵泡液G-CSF显著更低。当卵泡液G-CSF为20 pg/ml或更高时，顶级胚胎与非顶级胚胎的比例显著高于卵泡液G-CSF低于20 pg/ml的组（45%对20.45%，P = 0.007）。
单个卵泡液组成与相应体外产生胚胎的发育及其着床潜力相关。单个卵泡液G-CSF可能提供一种着床的非侵入性生物标志物，需要与体外观察一起评估，以选择用于移植的卵母细胞，进而选择胚胎。
