Gurcan Saban, Karabay Oğuz, Karadenizli Aynur, Karagol Ciğdem, Kantardjiev Todor, Ivanov Ivan N
Department of Microbiology and Clinical Microbiology, Faculty of Medicine, Trakya University, Edirne, Turkey.
Jpn J Infect Dis. 2008 May;61(3):223-5.
In this study, cultures of patients with tularemia were evaluated, and antimicrobial susceptibilities of two Francisella tularensis strains were tested by disk diffusion and E-test methods. A high-resolution multiple-locus variable-number tandem repeat analysis (MLVA) comprising six variable-number tandem repeat loci was applied to elucidate the genetic relatedness among Turkish and Bulgarian isolates which were isolated in a recent outbreak. The patients were diagnosed in two outbreaks in two cities of Turkey in 2005 and 2006. A total of 16 samples from 12 patients were cultured, and PCR tests were carried out on 15 samples that were positive in five lymph node aspirates and two soft tissue aspirates. F. tularensis was isolated from the lymph nodes of two patients. Aminoglycosides, quinolones, chloramphenicole, tetracyclines, nitrofurantoin, and rifampicin inhibited growth of the isolates. The Turkish isolates appeared to share a common MLVA pattern with one of the four Bulgarian outbreak genotypes.
在本研究中，对兔热病患者的培养物进行了评估，并通过纸片扩散法和E-test法检测了两株土拉弗朗西斯菌的抗菌药敏性。应用包含六个可变数目串联重复序列位点的高分辨率多位点可变数目串联重复序列分析（MLVA）来阐明在近期一次疫情中分离出的土耳其和保加利亚菌株之间的遗传相关性。这些患者于2005年和2006年在土耳其的两个城市的两次疫情中被诊断出来。总共对12名患者的16份样本进行了培养，并对在五份淋巴结抽吸物和两份软组织抽吸物中呈阳性的15份样本进行了PCR检测。从两名患者的淋巴结中分离出土拉弗朗西斯菌。氨基糖苷类、喹诺酮类、氯霉素、四环素类、呋喃妥因和利福平抑制了分离株的生长。土耳其分离株似乎与四种保加利亚疫情基因型之一具有共同的MLVA模式。
