Iwatsuki Masato, Uchida Ryuji, Yoshijima Hitomi, Ui Hideaki, Shiomi Kazuro, Matsumoto Atsuko, Takahashi Yoko, Abe Akio, Tomoda Hiroshi, Omura Satoshi
The Kitasato Institute, Japan.
J Antibiot (Tokyo). 2008 Apr;61(4):222-9. doi: 10.1038/ja.2008.32.
Enteropathogenic Escherichia coli (EPEC) expressing the Type III secretion system (TTSS) induced hemolysis of sheep blood cells. Using this assay, six structurally related compounds designated as guadinomines were isolated as inhibitors of TTSS-induced hemolysis by ion exchange column chromatography and HPLC from the culture broth of Streptomyces sp. K01-0509. Guadinomines A and B showed potent inhibition with IC50 values of 0.02 and 0.007 microg/ml, respectively, guadinomine D showed moderate activity (IC50: 8.5 microg/ml), while guadinomines C1 and C2 and guadinomic acid had no activity.
表达III型分泌系统（TTSS）的肠致病性大肠杆菌（EPEC）可诱导绵羊血细胞溶血。利用该检测方法，通过离子交换柱色谱法和高效液相色谱法，从链霉菌属菌株K01 - 0509的培养液中分离出六种结构相关的化合物，命名为胍丁胺类化合物，作为TTSS诱导溶血的抑制剂。胍丁胺A和B表现出强效抑制作用，IC50值分别为0.02和0.007微克/毫升，胍丁胺D表现出中等活性（IC50：8.5微克/毫升），而胍丁胺C1、C2和胍丁胺酸则无活性。
