胞嘧啶霉素，由链霉菌属KO-8119产生的新型抗球虫剂。I. 分类学、生产、分离以及物理化学和生物学特性。

Cytosaminomycins, new anticoccidial agents produced by Streptomyces sp. KO-8119. I. Taxonomy, production, isolation and physico-chemical and biological properties.

作者信息

Haneda K, Shinose M, Seino A, Tabata N, Tomoda H, Iwai Y, Omura S

机构信息

Research Center for Biological Function, Kitasato Institute, Tokyo, Japan.

出版信息

J Antibiot (Tokyo). 1994 Jul;47(7):774-81. doi: 10.7164/antibiotics.47.774.

DOI:10.7164/antibiotics.47.774

PMID:8071122

Abstract

Streptomyces amakusaensis KO-8119, a soil isolate, was found to produce a series of new anticoccidial compounds. Four active compounds, designated as cytosaminomycins A, B, C and D, were isolated from the fermentation broth of the producing strain by solvent extraction, silica gel column chromatography and preparative HPLC. Cytosaminomycins inhibited the growth of Eimeria tenella in an in vitro assay system using primary chicken embryonic cells as a host. No schizont in the cells was observed at concentrations ranging from 0.3 to 0.6 microgram/ml for cytosaminomycins A, B and C, and at 2.5 micrograms/ml for cytosaminomycin D.

摘要

天草链霉菌KO-8119是从土壤中分离得到的，被发现能产生一系列新的抗球虫化合物。通过溶剂萃取、硅胶柱色谱和制备型高效液相色谱从产生菌的发酵液中分离出四种活性化合物，分别命名为胞嘧啶氨基霉素A、B、C和D。在以原代鸡胚细胞为宿主的体外检测系统中，胞嘧啶氨基霉素抑制了柔嫩艾美耳球虫的生长。对于胞嘧啶氨基霉素A、B和C，在浓度为0.3至0.6微克/毫升时，细胞中未观察到裂殖体；对于胞嘧啶氨基霉素D，在浓度为2.5微克/毫升时，细胞中未观察到裂殖体。