Vezzulli Silvia, Troggio Michela, Coppola Giuseppina, Jermakow Angelica, Cartwright Dustin, Zharkikh Andrey, Stefanini Marco, Grando M Stella, Viola Roberto, Adam-Blondon Anne-Françoise, Thomas Mark, This Patrice, Velasco Riccardo
IASMA Research Centre, San Michele a/Adige, Italy.
Theor Appl Genet. 2008 Aug;117(4):499-511. doi: 10.1007/s00122-008-0794-3. Epub 2008 May 27.
We have developed an integrated map from five elite cultivars of Vitis vinifera L.; Syrah, Pinot Noir, Grenache, Cabernet Sauvignon and Riesling which are parents of three segregating populations. A new source of markers, SNPs, identified in ESTs and unique BAC-end sequences was added to the available IGGP reference set of SSRs. The complete integrated map comprises 1,134 markers (350 AFLP, 332 BESs, 169 ESTs, 283 SSRs) spanning 1,443 cM over 19 linkage groups and shows a mean distance between neighbouring loci of 1.27 cM. Marker order was mainly conserved between the integrated map and the highly dense SyrahxPinot Noir consensus map except for few inversions. Moreover, the marker order has been validated through the assembled genome sequence of Pinot Noir. We have also assessed the transferability of SNP-based markers among five V. vinifera varieties, enabling marker validation across different genotypes. This integrated map can serve as a fundamental tool for molecular breeding in V. vinifera and related species and provide a basis for studies of genome organization and evolution in grapevines.
我们构建了一张整合图谱，其来源于酿酒葡萄（Vitis vinifera L.）的五个优良品种：西拉（Syrah）、黑皮诺（Pinot Noir）、歌海娜（Grenache）、赤霞珠（Cabernet Sauvignon）和雷司令（Riesling），这五个品种是三个分离群体的亲本。在EST和独特的BAC末端序列中鉴定出的一种新的标记来源——单核苷酸多态性（SNPs），被添加到现有的国际葡萄基因组计划（IGGP）简单序列重复（SSRs）参考集中。完整的整合图谱包含1134个标记（350个扩增片段长度多态性（AFLP）、332个BAC末端序列（BESs）、169个EST、283个SSR），跨越19个连锁群，总长1443厘摩（cM），相邻位点之间的平均距离为1.27 cM。除了少数倒位外，整合图谱与高度密集的西拉×黑皮诺共识图谱之间的标记顺序基本一致。此外，标记顺序已通过黑皮诺的组装基因组序列得到验证。我们还评估了基于SNP的标记在五个酿酒葡萄品种之间的可转移性，从而能够在不同基因型间进行标记验证。这张整合图谱可作为酿酒葡萄及相关物种分子育种的基础工具，并为葡萄基因组组织和进化研究提供依据。
